We narrowed to 173 results for: GFP

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

Oct. 3, 2024, 1:15 p.m.

This month's Hot Plasmids articles features new plasmids, new antibodies, and a viral ticker tape system!

Nov. 14, 2023, 3:35 a.m.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

July 30, 2020, 1:15 p.m.

An introduction to gene, enhancer, and promoter trapping. Relevant examples of each along with pros and cons of each system.

May 2, 2023, 1 p.m.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

April 9, 2015, 3:29 p.m.

mNeonGreen is a bright green fluorescent protein. Along with mWasabi and mTFP, it can be used for traditional imaging, FRET, and multicolor imaging.

April 25, 2019, 3:01 p.m.

Tips on how to choose compatible fluorescent proteins for multi-color imaging.

Oct. 9, 2014, 3 p.m.

This guide on single chain fragment variables (ScFvs) covers how ScFvs are made, the advantages and disadvantages of using ScFvs, and several applications.

June 3, 2021, 1:15 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

CRISPR-Sirius is a new tool to visualize genomic loci using dCas9. It's brighter than its predecessor CRISPRainbow and unlike fluorescent in situ hybridization, can be used in live cells.

April 18, 2019, 12:53 p.m.

This Twelve Days of Antiboides, we're celebrating some cool new additions to our antibody repository and resources.

Jan. 6, 2025, 5:17 p.m.

A review of the types of engineered biosensors and how viruses can be used to deliver them.

July 6, 2023, 1 p.m.

Learn about SF-iGluSnFr, an updated glutamate biosensor from Loren Looger's lab.

April 7, 2022, 1:15 p.m.

Our February hot plasmids, including two new CRISPR tools, a protein ladder, and anti-FLAG frankenbodies.

Feb. 22, 2022, 2:15 p.m.

Learn about new plasmids at Addgene including AAV split base editors, Ace2-mScarlet voltage indicator, CyanoGate MoClo cloning toolkit, and CreLite.

March 3, 2020, 2:15 p.m.

Get advice on tagging optogenetics and chemogenetics receptors to ensure that your receptors express and/or localize to the membrane.

May 11, 2021, 1:15 p.m.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

June 27, 2019, 12:25 p.m.

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

April 28, 2016, 2:30 p.m.