We narrowed to 173 results for: GFP

...section. The GFP story has its own Franklin in Doug Prasher, who originally isolated the GFP gene but was...the identification of Green Fluorescent Protein (GFP) and its development into an essential tool in biology...

...Since the discovery of GFP over 50 years ago, the growing spectrum of fluorescent proteins (FPs) has ...repair pathway, a new user-defined DNA sequence (like GFP) can be inserted at the DSB. CRISPR has allowed scientists...toolkit offers C- and N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin...

...AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB...Recombinases Jonathan Ting New viral prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP...

...transcriptional activation events as bands of induced GFP-iPAK4 expressed under promoters like the tet-responsive...fluorescent signal in the ticker tape from cFos::GFP-iPAK4 within an hour. Analyzing the ticker tape images...to red at 12 h, and PMA activation induces cFos::GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted...

...constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with...result in Figure 1, an internal control, such as the GFP-expressing Plasmid B, could demonstrate whether the...transfection was not successful due to the absence of GFP fluorescence from the living, viable cells. This ...

... way. For example, if green fluorescent protein (GFP) was the reporter gene and green cells were observed...developmental stage you could infer that the gene which GFP was monitoring was being expressed in the same pattern...

...substitutions to LanYFP and the addition of the enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the... to 3 times brighter than the most commonly used GFPs and YFPs. Its excitation maxima is at 506 nm and...

...various combinations of protein tags (His, Avi, and GFP) as well as protease sites to facilitate protein ...’ end of the leader sequence. Using a variety of GFP and RFP fusions as their test genes, the researchers...terminators of varying strengths centered within GFP/RFP bicistronic reporters characterized in Cambray...

... / em 527 nm) can be imaged together but CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between...this set are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...

...binding scFv that’s fused to GFP. When the multiple copies of the scFv-GFP fusion bind the SunTag scaffold...scFv that’s fused to a fluorescent protein such as GFP. The HA frankenbody works just like an antibody-based...

...regulation. To create iGluSnFR, the Looger lab wedged GFP between two halves of a bacterially derived periplasmic...increase in fluorescence intensity from the inserted GFP. After characterizing iGluSnFR in vitro, showing ...

...each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs of...hairpin sequence (sgRNA-Sirius-8XPP7) to recruit GFP to the loci of interest. To determine how the sgRNA-Sirius...

...Antibody, Addgene gave to me…  A recombinant anti-GFP!    🎶On the third day of Antibody, Addgene gave ...

...interest (analyte), and accumulation of ATP activated GFP expression (receptor), then the transducer could ...plate reader, or any other instrument which measured GFP levels and converted that signal into a readable ...

...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...

...levels similar to that of a single AAV expressing GFP. The lab tested the base editors in vivo and achieved...voltage indicators, however, fall short compared to GFP-based sensors in terms of response and brightness...

...selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both sorted using FACS and isolated...allows translation of the reporter gene resulting in GFP signal. With the addition of standards, the assay...

... constitutive promoter upstream of GFP increased expression of GFP six-fold, but a stabilized promoter...

...protein of interest. The green fluorescent protein (GFP) and mCherry, two of the most common tags currently...article on the Addgene blog: Fluorescent Proteins 101: GFP Fusion Proteins – Making the Right Connection.   ...

... should contain a reporter DNA sequence (such as GFP) in the sense orientation, followed by coding sequence...et al. Nat Biotech 2003): In the presence of Cre, GFP expression is lost, allowing for rescued expression...