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...of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter so be... expression. The plasmids associated with this article can be obtained through Addgene: Rapamycin-inducible...course of gene activation using this system in the article cited below. Scientists can obtain dCas9-trCIB1...trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and...TOP2 constructs; see Supplementary Note 3 in the article for more info) are available. Furthermore, there...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here’s what you’ll find in this article: CRISPR-based editing in organoid Biosensors for...

...the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down. ...100°C. They published their finding in a bioRxiv article and in PNAS. Two other thermostable Cas9 enzymes...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here's what you'll find in this article: An improved cell-free system to examine biosynthetic...

...types of biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...

...a brief overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes...different AAV vectors into a brain region, the AAV particles will infect neurons at different ratios, making...and may be a form of transcytosis where the AAV particle is taken up on one side of the cell and released...cell. While there is always a risk that viral particles will diffuse long distances within the extracellular...

...more viral particles. Eventually the cell lyses releasing the newly formed viral particles that can infect...are packaged into replication-incompetent phage particles with assistance from a ‘helper’ phage prior to...

...too large to be effectively packaged into AAV particles for delivery into mammalian cells, especially ...mRNA to facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA...SEND for gene transfer with pseudotyped viral particles, expanding options for CRISPR or other gene delivery...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... the Biggiephage clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1...

...to update our post. From Mice to Men When this article was originally written, the main focus was on editing...technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to the liver...disease approaches targeting cholesterol, these particles were an obvious delivery choice. In sum, CRISPR...

...stock, followed by resuspension of the collected particles in a smaller volume. Always remove packaging cell...reagents typically enhance the adsorption of a virus particle to a target cell by reducing the repulsive electrostatic...an accurate count of the number of infectious particles in a given viral stock will give you a sense of...

... modified in the golgi and then transported in vesicles via the secretory pathway to their subcellular...cycle, proteins may be transported through the vesicles to the lysosome where they are finally degraded... hope you find the resources discussed in this article useful for your next localization experiment and...

...dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes to FP... et al. "Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by ...

...examples: experiment or analysis. In the rest of this article, we'll explain the thinking behind the tool, its...do this internally, but they are not available publicly. Furthermore, we did not find any tools which ...the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis...

...highlight some of the reagents that have been made publicly available through Addgene.  What makes CRISPR... Individual gRNAs are designed in silico using publicly available gRNA design software and synthesized...derivatives of Cas9. Several CRISPR libraries are publicly available through Addgene, with more being added...

...1,2), and cell types used for packaging viral particles (6), can influence vector performance. Open science...citable DOIs through DataCite before being made publicly available through the Data Hub. These minimum ...of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent its occurrence...

...genomic, or DNA fragments from agarose).    In this article, we will go through the basics of four methods ...milk-like appearance. To make DNA stick to the glass particles in glass milk, gel slices are first placed in ...stick to the glass. After binding DNA, the glass particles are pelleted by centrifugation and washed before...

...checked out Gal's blog, you should! He's got great articles and insight on the newest tools and techniques...fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. ...Max Juchheim contributed to the writing of this article. ...

...preps in the repository through our Hot Plasmids articles. Today, it's time for another edition of Hot Plasmids...and targeting components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for...   Figure 2: Modular features of DIRECTED particles where the fusion and targeting components are ...

...study showed that out of 238 published scientific articles, only 46% could be reproduced. In a recent Nature...notebook requires time and knowledge. In a recent article, we translated our knowledge of electronic lab ...what exactly your lab needs from an ELN (the full article is available here):   Does your lab need to keep...