Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 362 results for: Numb

Showing: 61 - 80 of 362 results

  1. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...be excited many times, thereby producing a large number of emitted photons before they are destroyed. ...decreasing the power of the excitation light reduces the number of excitation/emission cycles per unit of time,... are performed in different ways. We describe a number of issues with some of the experimental designs...

  2. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...least twice, and sometimes more depending on the number of features we need to verify. Determining which...is critical for the efficient handling of large numbers of samples. When we grow overnight cultures for...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your life...

  3. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...calculate the particle number: OD260 reading x dilution factor x 1.1 x 1012 particles = number of particles per...purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions is determined ...

  4. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...catalog number, or at least the plasmid name in your initial query. This can reduce the number of messages...questions about an Abcam antibody. Have your catalog number ready. I've attempted to answer several emails ...

  5. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... years (we now have over 60,000 plasmids!), the number of sequencing results we analyze as part of our...sequences (nr)” database as it contains the largest number of protein sequences. Just below the BLAST button...isoforms. In addition to our favorites, there are a number of sequence alignment tools available. Try these...

  6. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...western blot. Standard curves are critical for a number of popular scientific applications, and the quality... is because standard curves vary depending on a number of factors, including the user, equipment, assay...accurately measure.  Dynamic range depends on a number of factors, including the dilution series of the...

  7. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... requests by date. Parentheses depict the total number of plasmids ordered for each paper. Rough estimation...affected by the COVID-19 pandemic, we compared the number of plasmid orders and new COVID-19 cases for each... our data: We choose to let yx(t) represent the number of unique PIs or labs placing orders for paper ...

  8. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... Our scheduling was in part defined by both the number of participants and the standard academic calendar...logistics Once you decide on the topic, there are a number of administrative and practical decisions to make...scheduling the second and other subsequent meetings. The number of participants, breadth of the discussed topic...

  9. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...were derived from. They also transduced a higher number of neurons overall (GFP+ cells that also stained...doses of virus are used in order to decrease the number of neurons that get labeled, neurons are more likely...tTA), is injected at a high dose to maximize the number of cells that are labeled. Part 2: An AAV expressing...

  11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...sample has a limited number of fluorophores and each fluorophore can emit a limited number of photons before...protein fusions to gene-edited cells can decrease the number of fluorophores, compromising the photon budget...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...to overexpress and purify proteins can face any number of technical challenges depending on their protein...might consider junk protein. Though there are a number of chemical and peptide-based proteosome inhibitors...of this cleavage depends on the identity of X. A number of other proteases are available, but scientists...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...proteins. These iterons vary in their sequence and number and can alter the efficiency of replication in ...Regulatory elements within the ori also control copy number and stability, which influence the plasmid’s ability...self-transmissible plasmids may have high copy numbers and reduced metabolic load (Meyer 2009). ...

  14. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...cells than the number of plasmids in the library.  Spinfection If you have a large number of cells to transduce...lentiviral preps are generally aliquoted to reduce the number of freeze-thaw cycles needed when using the virus...

  16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...sharing The EDGE Program is designed to expand the number of systems that can be studied by providing support...Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually high level of RNA...homologous recombination efficiency to reduce the number of plants you need to grow to get a recombinant...

  17. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...journals Current Biology (2,500 views), Journal of Number Theory (1,519 views), and Gastroenterology (1,046...., learn why water freezes) there was a growing number of online research abstracts. With journals in ...Noorden, Richard. "Interdisciplinary research by the numbers." Nature 525.7569 (2015): 306-307. 3. Spicer, Scott...

  18. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...advised me on how to alter my intro to reduce the number of potentially hostile posters (this advice included...moderators did not delete or suppress were the large number of scientist posters with serious career dilemmas...associated with my LinkedIn profile. There are a number of ways you can build up your own expertise and...

  19. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...’s wise to quickly assess whether a significant number of cells have been edited. For many experiments...population. You can additionally calculate the minimum number of clones you would have to screen to identify ...occur, allowing you to Sanger sequence a manageable number of the higher probability locations. If you’re ...

  20. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...Unfortunately, even when universities have simple postdoc numbers sorted out there can be gaps in knowledge of demographics...and some that stuck out to me were: Decrease the Number of Postdocs De-incentivize going into PhD programs...services based on what they're spending. Increase the number of science masters programs to prepare scientists...
Showing: 61 - 80 of 362 results