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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 221 results for: PORA

Showing: 61 - 80 of 221 results

  1. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...2024). They developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7...high microglia-specific gene expression when incorporated in an AAV vector (Figure 5). Efficiency and ...

  2. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Xanthomonas campestris   In addition to incorporating broad host range replicons, recombinant vectors...can be expressed. For example, scientists can incorporate interchangeable antibiotic resistance cassettes...

  3. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...tumefaciens, a plant-infectious bacteria that can incorporate foreign DNA into plant genomes. Eukaryotes are...stable transfection, DNA from the plasmid is incorporated into the host cell’s genome, so no special sequence...

  4. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...synthesis polymerase known to preferentially incorporate A bases across from abasic sites. This approach...a template — in this case, encouraging it to incorporate a new base across from the abasic site before...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...ICSS can now be done with neuronal cell-type and temporal precision. In ICSS, a test chamber is used in ...more precisely characterized with spatial (CA1), temporal (real-time inhibition), and behavioral (remote...

  7. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... scientists to easily control the level and spatiotemporal expression of a gene of interest. This type...method that uses heat induction to control the temporal expression of different transgenes, the Stainer...

  8. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...version of my manual, I added new chapters to incorporate the experiences of the people that provided me...interviews. We didn't have Skype in 2004. I also incorporated example documents. This part has generated a...

  10. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...lengths because extension is terminated upon the incorporation of a ddNTP. Researchers perform four different...activity as fluorescently labeled nucleotides are incorporated, a method called pyrosequencing. The drive for...

  12. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...individual types of GPCRs with greater spatial and temporal control than is achieved by traditional pharmacologic...unknown.      Figure 3: Chimeric OptoXR incorporating rhodopsin undergoes a conformational change ...

  13. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...obstacle, a positive selection cassette can be incorporated, and used during the differentiation process...homologous recombination, which results in the incorporation of your specific edit into the host genome. ...

  15. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...Ridley Scott wonder what would happen if these corporations would extrapolate genetic engineering to exploit...which are genetically engineered by a powerful corporation to serve as slaves.  Meganucleases In 1989 the...

  17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...in the ribosome, resulting in site-specific incorporation of the attached fluorophore. However, since ...Ebright, and Richard H. Ebright. "Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...their enhanced TANGO arrestin recruitment assay incorporating 314 codon-optimized GPCR sequences for human...MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand DNA (ssDNA) oligonucleotides...

  20. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...The Frequency Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that...
Showing: 61 - 80 of 221 results