We narrowed to 554 results for: PPL

...technique published every week! One of the newest applications is a colorful system that uses fluorescently...activity, catalytically dead Cas9 (dCas9) has many applications, the most well-known being transcriptional activation...characterizing the 4D nucleome. Future modifications and applications Ma et al. developed this technique using standard... et al. anticipate that the technique will be applicable to studies of cell cycle progression, epigenetics...to external stimuli. It could also have major applications to cancer, for example, the visualization of...

...tag to remain on your protein for downstream applications Below, we describe a few of the most common...not to perturb protein function for downstream applications. Tag cleavage must be performed after primary...also provides a visual detection mechanism via applications such as western blot and immunofluorescence....dimerizes in solution, which can affect downstream applications. Also, purifying GST by affinity chromatography... are generally best suited for their primary application. Ready to start tagging? We are here to help ...

...different CRISPR applications in plants.     Transgenic yeast as source of dietary supplements Dr. Mrinalini...well as developing yeast as source for dietary supplements! Development of a bacterial expression system...Vietnam Linh Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  ...either.  Test tubes aren’t cheap everywhere Lab supplies and equipment are more precious in less resourced...Labs does an amazing job shuttling unwanted lab supplies to places that really need them.  When I was in...stop me. I only had to replenish my glass milk supplies every 10 years or so. Actually, I was happy to...

...isolating the cell line all the way to modern applications. What the HEK is it?   HEK cells were first...cell biology, there are thankfully many other applications that they are useful for! What the HEK is it...cell line and we will highlight some of those applications here.   Virus production HEKs are the go-to ...proteins at high levels. In a case where clinical applications of recombinant proteins are necessary, HEKs ...significantly reduces the cost of production. Research applications There are two areas of research where HEK cells...

...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,...for, we wanted to be certain that this supplementation strategy would have no effect on the purity or infectivity... we will be spending less time and resources resupplying our current inventory and more time providing.... "Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate." Biotechnology...

... be applied to dozens of other published FRET-based sensors. For example, his team is now applying it ...Because they are chemical compounds, they have to be applied or injected, and they don’t always end up where...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be... large dynamic range,” he says. Merkx recently applied this “trick” to the development of additional color...

...tool that can be used for a variety of research applications. Within the immune system, antibodies are produced...antibodies to bind proteins is useful for research applications as well because they allow scientists to target...150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies You can use antibodies for experiments...Let’s dive in and take a look at some of these applications. Western blot Western blots are used to detect... by binding to cell surface receptors. These applications are typically used for investigating signaling...are out there, and how many different research applications they can be used for. Addgene’s Antibody Plasmid...

...information they’ve absorbed if they don’t get a chance apply it in an interactive setting. The problem is…how... as I try being compelling, interactive, and application-driven to boost curiosity, I have never experienced...they would use their logic and strategy skills to apply those concepts and understand how therapeutic strategies... BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, microbiology...opposing team. In the end, the students had fun while applying most of the concepts required by the course and...

...research box, send out some feelers, apply to programs, and apply for travel grants. Good luck and happy...visiting positions within defined labs which you can apply to. These structured types of placements often have...are your options if neither of these situations apply to you? Once you’ve identified one or more labs ...mentor can help get the ball rolling! If you are applying to a structured program, they likely have guidelines...

...together a grant application? Are you familiar with the publishing or patent application processes? How ...How can you stand out among all the candidates applying for the position?   Here are a few things to consider...makes sense for your skills, and the job you are applying for. Also remember that a resume is typically ...have done is closely related to the job you are applying for, in which case talk about your research, as...

... plasmid preps are the same. Your downstream application will dictate how much DNA you need to recover...recover and the level of quality required. Some applications require very little DNA and you can get away with...with slightly lower quality preps, while other applications require large amounts of high quality DNA. Figure... a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self explanatory and...settings, but when it comes to low-throughput applications, the choice is largely a matter of preference... 96-well plate options for medium-throughput applications. For high-throughput options, several manufacturers...

...and deliver any cargo you fancy (size limits do apply, however.) Why use a virus? Viruses are essentially...low immunogenicity which makes them ideal for applications with whole organisms. Finally, viral delivery...delivery is typically straightforward across applications, while electroporation of a human, for example, isn... (~4.5kb From basic science to clinical applications, viruses are true bench to bedside tools. Keep...our upcoming blogs on virus safety and viral applications to help you start (safely!) thinking about the...

...definition of “problem solving skills” is the ability to apply the scientific method to problems in a variety of...strong problem solving skills is just a matter of applying that framework to the myriad challenges we encounter...ask them to explain their logic. Or you can try applying the scientific method to everyday problems in ...to practice problem solving skills and learn to apply them flexibly in a range of contexts. As such, scientists...remember that you have these skills when you start applying and interviewing for new positions. Highlighting...

...population. While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these...will not necessarily express EnvA unless it is supplied in trans. Monosynaptic RABV spread can be achieved...transsynaptic spread can be controlled by selectively supplying the G protein in trans only to starter cells (...replication will then occur and the G protein (supplied in trans) will be incorporated into the viral ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...

...review how these indicators work, their general applications, and why viruses are essential to their use ...improvement. These sensors still have many useful applications, though, and if you’re in the market for a VSD...tissues depths (1 mm), important for certain applications such as in vivo tissue imaging.   Figure...your cell type of interest. For in vivo imaging applications, the ability to direct your voltage sensor selectively...genetically encoded voltage indicators advance their applications in neuroscience. Curr Opin Biomed Eng. 2019....

...well as suitable applications. Expression System Components of System Application Mammalian expression...CRISPR mammalian expression systems, the typical applications for each, and potential delivery methods.   ...Select the appropriate CRISPR tools for your application: Wildtype Cas9 or Cas9 nickase are appropriate...or more sgRNAs and Cas9 are required for any application. If you want to include a homology directed repair...

...improvements to CRISPR technology since its initial applications for genome engineering in 2012.  Many of these...particular interest since blue light has limited applications in vivo. UV and blue light is readily absorbed...allowing DNA binding and cleavage. This technique was applied to create photoswitchable spCas9, dCas9, and S...., allowing the release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated...Photoactivatable moiety Cas9 variatn CRISPR protein application Split Systems / Light-induced Dimerization ...continue to evolve and can’t wait to see what cool applications you use these for in the future! References...

... and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA ...reading results from a NanoDrop. The principles will apply to similar instruments from other companies, like...sample will have minimal impact on downstream applications, but they can impair your ability to get an ...be sure it is compatible with your downstream applications and that you use the same solution for blanking...A230 ratios in RNA preparations on downstream applications. Qiagen Gene Expression Newsletter. Issue 15...

...production system may depend on what downstream applications you are planning, cost, and the amount of protein...accurate concentration to give your downstream applications the best chance at being successful.  Using ...they bind their antigens specifically. Possible applications for validation include western blot, immunohistochemistry...some antibodies are better suited for certain applications. An antibody that performs well in immunohistochemistry...use! You can learn more about common antibody applications in our Antibodies 101 blog series.  Resources...