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We narrowed to 289 results for: Pol

Showing: 61 - 80 of 289 results

  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ... and surveyor. Q15: I used DNA polymerase Takara Ex Taq™ DNA Polymerase for my genomic PCR, but couldn't...In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well. Some groups have ...your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure 4 of the Cong et. al (2013...

  2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ... Traditionally, they have been made as either polyclonal antibodies or monoclonal hybridomas, but those...those techniques have several drawbacks. Polyclonal antibodies produced in animals may be variable among...& Plückthun, 2015a).   Figure 1: (Top) Polyclonal antibodies are produced in animals and consist...Recombinant antibodies offer numerous advantages over polyclonal antibodies and traditional hybridomas.  First...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... both vector amplification with low fidelity polymerases and the cost of synthesizing longer oligos, which...simple cloning procedures. Use high fidelity DNA polymerase This will minimize random errors during PCR: ...aliquots of PCR master-mix, containing buffer, polymerase and dNTPs, can be stored frozen: just defrost...mutagenesis by placing homologous ends on DNA using polymerase chain reaction." Biotechniques 10.1 (1991): 62...

  4. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...slow the polymerization of formaldehyde.  Note: Formaldehyde in solution will naturally polymerize and lose...paraformaldehyde (PFA) as the fixative. PFA is a polymer of formaldehyde that, on its own, is not a fixative...PFA is dissolved in solution and heated, it depolymerizes, producing formaldehyde. Often people will call...

  5. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...biomolecules to the gel and add gel components to polymerize. Step 3: digest the sample to allow for even ...components penetrate the sample, how well the gel polymerizes, and the level of digestion. All of these factors...of epitopes during sample anchoring and gel polymerization - issues that can be minimized through tweaks...tweaks to these steps. But even under ideal polymerization and digestion conditions, loss of signal often...

  6. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    .... Arguably the simplest affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function...the tag size quickly – modifying the length a polyhistidine tract does not greatly alter the size of the...used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification Very small...Solubility and Affinity Abundance of charged and polar residues improves solubility; good for antibody-...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...of the sample to that of a standard curve to extrapolate the concentration of antigen in the sample. A... present in the sample and is calculated by extrapolating from the standard curve. Because the primary... present in the sample and is calculated by extrapolating from the standard curve. Scientists often opt... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene...

  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...positively charged polycation, polybrene. Interestingly, one study found that alternative polycations such as DEAE-dextran...efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14...

  9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... sample and subject to isothermal recombinase polymerase amplification (RPA) using a commercially available...reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that...transcription. This is then amplified by PCR using Taq Polymerase and then transcribed to provide RNA substrates...instead of RPA. Unlike RPA, this technique uses Taq polymerase which is widely available and less expensive....

  10. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...developed to quantify RNA transcription by RNA Polymerase III. By expressing Corn under promoters for transcripts... transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence...effect of mTOR inhibition on the activity of RNA Polymerase III. Red and orange fluorescent aptamers Corn...PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription using a photostable RNA–fluorophore...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... biology and biotechnology purposes.  Once a polypeptide is biotinylated (more on this below) it can be...Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated ...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...

  12. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...single stranded regions with homology to anneal a polymerase, to fill in the gaps a DNA ligase, to seal the...than 200 nucleotides before the annealing and polymerization steps could occur. Secondly, it does not work...instead of using the exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or ...methods that have been described--CPEC (circular polymerase extension cloning) and SLiCE (Seamless Ligation...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are...Na+) against the concentration gradient and hyperpolarize the cell (Mattis et al., 2012). Bicistronic ...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they... A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES is an optogenetic tool developed for bidirectional...

  14. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters... sensor, membrane depolarization increases the BRET signal whereas hyperpolarization decreases the signal...

  15. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...join any fragments end-to-end without the exo/polymerase generation of annealed complementary regions....and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping enzyme superfamily of...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

  16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...binding to the template strand (strand used by RNA polymerase II for transcription) are more effective for ...editing! Pooran Dewari is a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine...immunity and mammalian genome editing via RNA polymerase-mediated dislodging of Cas9 from double strand...

  17. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...Updated March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name!...participation in suggesting names and voting in our poll. Now, we'd like you to meet..... *drumroll* Aavery... out this form here linked below. We’ll keep the poll open until February 28 5PM EST and we’re excited...

  19. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ... uses do you foresee for this tool? Howarth: Polyproteins are useful for mechanical study of proteins ...proteins by atomic force microscopy (AFM). Maybe polyantibodies could help in the capture of other rare cell ...References: SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...microelectronic fibers made from thermoplastic polymers that can work with all the bells and whistles....called thermal drawing, essentially where blocks of polymers are heated up and pulled to a desired thickness... small molecules.   Fun fact: A 1 cm block of polymer can generate many meters of fibers!     ...
Showing: 61 - 80 of 289 results