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We narrowed to 468 results for: SEC

Showing: 61 - 80 of 468 results

  1. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ... entirety of each day doing the experiment. The second way, it will take you 5 days to complete the experiment...advice from above, you should probably take the second option. Even if the full experiment fails, you’...need to do. In most cases, I would recommend the second option for the experiment above, but it’s also ...them. That’s why, as I’ll discuss in the planning section below, it’s important to dedicate specific amounts...

  2. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...annealing and polymerization steps could occur. Secondly, it does not work well if the ends of the fragments...fragments have stable single stranded DNA secondary structure, such as a hairpin or a stem loop (as might...vector where no other methods were available. A second example of using Gibson Assembly along with CRISPR...controlled by the presence or absence of dNTPs. Secondly, in Gibson assembly a ligase is added to repair...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... by del Valle Rodriguez et al.   LexA/lexAop A second binary system is LexA/lexAop, developed by Lai ...labs has refined the Q system and introduced the second generation QF2 activators. These activators solve...Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16, the trans-activator of herpes simplex...2843425.Takara Bio. Tet-On and Tet-Off systems: second generation. https://www.takarabio.com/products/...

  4. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...library of common parts to get you started quickly (Fonseca et al., 2019). Recently, the toolkit was used to...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no....Crossref, https://doi.org/10.1016/j.xplc.2020.100135. Fonseca, João Pedro, et al. “A Toolkit for Rapid Modular...

  6. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida...plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith Joung’s lab recently found ...efficiency. Read the paper in Nature Find the SECURE base editors All-in-one AAV delivery of Nme2Cas9...

  7. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... toolkit SECURE adenine base editor Earlier this year, Keith Joung’s lab developed SECURE cytosine base...compared to rat APOBEC1. Now, they’ve engineered SECURE adenine base editor variants using a protein-truncation...

  8. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...breaking month and to find other posts that deserve a second look. 5 Great Apps for Lab Life This very useful...mention any apps you’ve found useful in the comments section to make this post even more useful to your colleagues... attention to detail when writing the post. The second post, “Running For Rare Disease, Running for FOP...

  9. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...This is the second in a 5 part series on Management for scientists. This second installment in the Management...providing feedback pretty quickly.   This is the second in a 5 part series on Introduction to Managing ...

  10. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...proceeds through the various sections, the trainee speaks first for each section, preventing the meeting from... - let us know your strategies in the comments section!   References 1. Yearly Planning Meetings: Individualized...

  11. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...education experience (co-op) is what has brought me, a second year bioengineering student, to Addgene. I am currently... completing their co-op at Addgene. This is her second contribution to the Addgene blog.      More resources... Tips for Writing a Good Cover Letter 7 Tips to Secure a STEAM Internship This Summer  ...

  12. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    Published
    March 10, 2016, 3:30 p.m.
    ...collections. Addgene's "How to cite this plasmid" section Anytime you use a plasmid from the repository, ... that you can put straight into your reference section. Why do this? Not only does citing plasmids from...have come through our doors in our “Hot Plasmids” section. We’ve also added the Plasmid of the Day feature...

  13. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...primarily as protein purification tools (and can have secondary functions as detection aids.) When a single protein...performed after primary purification and requires a second clean up step to remove the cleaved tag from solution...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with ...

  14. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ..., who let me know in March that I successfully secured an internship at Addgene! I was eager to accept...reached out to Amy with exciting news. I managed to secure a virtual summer program, teaching me the basics...workspaces, as well as complete the lab safety and cybersecurity training before stepping foot into the lab itself...

  15. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ... in and they asked me, 'How do you feel about dissecting a cat?' And I’m like, 'Well, I’m here so let’...to pour a gel [for] electrophoresis or doing a dissection video. I did that in January. This involves going...s a gel electrophoresis tray in there. Or the dissection specimens.” And since the eScience warehouse ...

  16. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...kb. ITRs are inherently unstable due to their secondary structure, palindromic nature, and high GC content...typically fails to sequence through ITRs due to their secondary structure and high GC content, but some companies...sites, and then excise the sequence between the second pair. The final result is a permanently flipped...

  17. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...considerable number of researchers, I had high hopes of securing tenure in a British or Irish University where ... courses taken during my PhD and Undergraduate sections and brought in more focus on the list of conferences...towards me as a product and not me as a scientist. Second, I updated my LinkedIn profile, matched it with...

  18. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...protein secretion, making harvesting recombinant proteins a cinch. This is because it doesn’t secrete its ...proteins, making the recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's...option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated against...mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP [N86/8R] page for more details....

  20. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...Xin has been able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin...consuming in the first case and nonspecific in the second. Fatima therefore set out to design and produce...the amount of HMO originally in the sample. The second sensor is a fusion protein consisting of sfGFP ...
Showing: 61 - 80 of 468 results