We narrowed to 491 results for: Vit;

...targeted cells makes it possible to control neuronal activity with a specificity far greater than the one that...the axon’s membrane, thereby controlling neural activity (Bernstein and Boyden, 2011; Mei and Zhang, 2012...light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al., 2017). How to get...specific wavelengths, selectively turning neuron activity on or off (Atasoy et al., 2008; Taylor et al.,... this question, mapping and characterizing the activity of the DA neurons of the VTA. Using in vivo optogenetic...genetically targeted optical control of neural activity." Nature neuroscience 8.9 (2005): 1263. PubMed...

...depth and creativity of your work, while offering mental health benefits by encouraging activities beyond.... Widening your search The first step towards inviting serendipity into your research lies in expanding...lab research and gain fresh perspectives. Such activities foster a mindset that embraces the unexpected...openness to unexpected possibilities, you not only invite serendipity into your research but also cultivate...

...DNA guide impaired NgAgo activity, with three mismatches abolishing activity. One-guide faithful NgAgo..., showing that this protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately...RNA guides must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure...HDR, which could lower HDR efficiency. Higher sensitivity to off-targets More research is needed to compare...

...what is known as the tumor microenvironment - a vital atmosphere created by the cancer cells to shield...from attack. It is a region of incessant chemical activity, and consists of other cell types, especially ... immune cells that restrict the production and activity of other effector immune cells. TGFβ is also known...death ligand (PD-L1) that inhibits the cytotoxic activity of CTLs and NK cells. These cells would normally...when strengthened through efforts to boost the activity of the immune system, the therapeutic ability ...like CD19 on tumor cells and exhibit cytotoxic activity. CAR-T cells have consistently shown promise in...

... been used in immunology for in vitro screens of immune cell activity. However, a CRISPR-based tool for...microbiome and diseases, the immune system, and other vital systems in the body. When a microbe travels through...thereby serves as a sensor of miRNA presence and activity in a given system. If an miRNA is present, it ...to HEK293FT and HeLa cell lines. When the miRNA activity profile for a given cell type is already known...

... reversibly regulation of endogenous signaling activity in cells.  Let’s shed some light on the key components...AsLOV2 at a spot in the nanobody that preserves the activity of both AsLOV2 and the nanobody. As a proof-of-principle...OptoNBs The first application for OptoNBs takes us in vitro: light-controlled affinity purification of unmodified... could bind and block a receptor or a ligand’s activity, then a flip of a light switch could control a...Learn about using nanobodies to control protein activity Read about RANbodies, reporter nanobody fusions...

...would be ideal for such AAV packaging, but their activity in mammalian systems has been in question. Three...and making strategic mutations in the DNA binding cavity of Cas12f. The CasMINI system is less than half...The “V4” version (named CasMINI) had the highest activity for gene activation  and base editing, whereas...for its small size. They were able to increase activity and decrease the gRNA size by intensively remodelling...editors and a gRNA down-sized by almost 40%. The activity of Cas12f1 with these modified gRNAs is comparable...

... ability to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect... box, it will send an unpersonalized, generic invitation. Don’t try to connect from the LinkedIn phone... app either as this also results in a generic invitation. 3) Connect with people you have really talked... with this and as long as you personalize the invitation with your intention for connecting, this is probably...

...available with improved kinetic and sensitivity parameters. Sensitivity and Localization The calcium concentrations...unnecessary calcium buffering and to increase sensitivity), and is a great FRET sensor option if you have...populations of neurons in vivo to study circuit activity. CaMPARI is available for neuron-specific expression... Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically encoded calcium indicators. Cold...

...minimize the risk of cross-reactivity. To further limit species cross-reactivity, try to find secondary antibodies...And make sure to check for any reported cross-reactivity when planning your experiment.  When probing ...antibodies increasing specificity and reducing cross-reactivity. For example, if you are using mouse IgG2a and...-adsorbed secondary would limit species cross-reactivity and therefore reduce the background staining ...

...in the helical structure of spider silk, or the activity of a protein in a synapse. What kinds of tools...to measure specific small biomolecules or gene activity are often intramolecular probes, as the linker...protein-protein interactions, measure the concentration or activity of small molecules, detect cellular processes ...spring”) (Meng et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many ...Perspective of Single-Molecule FRET Biosensors and Intravital FRET Microscopy." Biophysical Journal 111.6 (...

... understand their activity and visualize the effects of alterations to this activity. Learn more about...protein-protein interactions, visualizing promoter activity, and much more. Read on to learn more about GFP...additional co-factors, substrates, or enzymatic activity – it only requires the presence of oxygen during...almost completely required to maintain fluorescent activity; very little truncation is tolerated, however,...

... RNA targeting system, Cas13, the "collateral" activity of the enzyme can lead to off targets and result...protein, and a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature CRISPR...:spacer duplex without detectable non-specific activity. Its major advantage is the significantly reduced...thousands new integrases with efficient integration activity in human cells. Moreover, they optimized the specific...engineering, enabling even more efficient integration activity. They now had a system that could integrate genes...

...transcriptome” studies, in which, for example, the activity of all genes in response to an experimental treatment...engages existing gene regulatory mechanisms to block activity and does not work easily in zebrafish.) Morpholinos... stages and reconstructed the patterns of gene activity and cell divisions that coordinate development...neuroscience investigations. For example, neuronal activity can be tracked in whole brains of swimming, actively...Zebrafish are also amenable to manipulation of brain activity: optogenetic approaches can be used to determine...

...system, they were able to monitor lacZ activity over 48 hours in vitro without antibiotic selection. In a ...processes and an antitoxin that suppresses the toxin’s activity. The key to these systems is that the toxin is...death. Conversely, CcdA binds to and blocks the activity of CcdB. Applications of ccdB in plasmid cloning...systems consist of two enzymes that have opposing activities, it is not surprising that they also play toxin-antitoxin...

...workplace is inevitable, but having who can help resolve these conflicts is not inevitable. When you know...operations and keeping our customers happy, but inevitably there will be gray areas that either a) overlap...meet. Most people can’t maintain that level of positivity past the age of five. But it does mean that even...competing, or accommodating, if you find yourself gravitating most frequently towards these approaches, you...

...for with in vitro display methods such as phage display (Bradbury et al., 2011). In vitro selection avoids...sequences are expressed as scFvs and screened with in vitro assays to select for strong binders. Then the sequences...2011) Beyond natural antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245–254 ...of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced...

...phage display assays, ubiquitination activity assays, toxin activity, small molecule binding, growth rate...discover unexpected epistatic changes in protein activity. See Natarajan et al for an example of this. ...Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis Protocols, pg. 103-109. PubMed PMID:.... Stephany, and Stanley Fields. “Measuring the Activity of Protein Variants on a Large Scale Using Deep...

...few minutes, I just need to split some cells.” Inevitably, this task would take longer than I thought, ... myself flexibility by never placing too many activities too close to one another. Monitoring task progress...) and, at the outset of a task, I outline the activities that are needed to accomplish these goals. Practically... of each day to remind myself of the specific activities that I need to work on. Throughout the day, I...during the times you’ve blocked out for other activities. This is also part of why I do much of my writing...

...other proteins, it may still block the function or activity of the tagged protein because of its size. This...There are several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration is the most...brightness, photostability, maturation time, and acid sensitivity of various fluorescent proteins (Botman et al...Determining other aspects of functionality (catalytic activity or interactions) can be challenging. If a fusion...fusion proteins: random insertion of GFP with an in vitro transposition reaction." BMC neuroscience 3.1 (2002...