We narrowed to 348 results for: abo.4

...Nano-lanterns, Takai et al. continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) ...improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color fluorescent imaging! These...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...Additional Resources on the Addgene Blog Learn about Photoactivatable Fluorescent Proteins Check Out ...

...bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into galactose and an insoluble blue pigment (4-chloro...antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening your cloning... plasmid is a great start when cloning, but how about being able to choose those that contain plasmid ... a widely used method to do just that! (To read about other screening strategies, check out our screening... called α-complementation. The system described above was put to practical use in the following way. Scientists...

...have a lower false positive rate than real-time PCR (4). Restriction mapping and modern sequencing Restriction...Nonradioactive Probes (2007): 205-226. PubMed PMID: 17332643. 4. Haruyama, Naoto, Andrew Cho, and Ashok B. Kulkarni...Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID:  25743089 . PubMed Central PMCID:...abundant in a sample. Whereas Southern blotting is labor intensive and requires a large amount of high-quality...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...PMC4411503. Resources on the Addgene Blog Learn More about Restriction Cloning Get Tips on Analyzing DNA Sequencing...

... they do for yes-no readouts.      Figure 4: A histogram plot for a smeary antibody readout. The... four populations in the histogram plot in Figure 4 compared to the dot plot in Figure 2. Quantification...interest. Created with biorender.com. The plot above is for a smeary expression and is quite different.... Additionally, you can gate off the histograms above, allowing you to visualize your data as a dot plot...

...UAS system, and you can read more about them in review articles (2–4). You can also take a look at my ...Genet. 20, 384–391 (2004). PubMed PMID: 15262411. 4. T. D. Southall, D. A. Elliott, A. H. Brand, The GAL4...

...additional anchoring domains – GPI and SGZ. Fig: 4 Sequence schematics for PDGFR, GPI, and SGZ display...constructs. Image adapted from Aggarwal et al… Figure 4. PDGFR is a C-terminal fusion to the PDGFR transmembrane...Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors A Practical Approach...

...VI-B, and VI-D being the most common. Figure 4: Overview of the general CRISPR type VI mechanism....Elements of Acidithiobacillia. The CRISPR Journal, 4(5), 656–672. https://doi.org/10.1089/crispr.2021.0051...competition between plasmids. Nucleic Acids Research, 48(4), 2000–2012. https://doi.org/10.1093/nar/gkz1197 Samai... type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013...Class 1 is the most abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly...CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions, and more...

... positions 4-7. ABEs 6.3, 7.8, and 7.9 display slightly wider editing windows of position 4-9, although...with a 14% indel rate, while ABE7.10 modified only 4/12 off-targets with a frequency of 1.2%. Although ...editing strategies are there? CBEs and ABEs can induce 4 out of 12 possible base-to-base changes. The other...cytidine deaminase fusions. Nature Biotechnology, 35(4), 371–376. https://doi.org/10.1038/nbt.3803 Koblan...Base Transversion Editors Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines... Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine base editor with improved...

... for Twitter pros as well, like tip #4: “Follow people for about a week and then unfollow those who do...employers and to arrange informational interviews. 4. “Tips for Scientists Using Social Media" tip sheet... advantage. It wasn’t until I wrote a blog post about a research paper that explored how scientists connect...saw a request for an Addgene guest blogger post about colony PCR, I signed up immediately. I had used ...science communication skills, while also learning about new scientific developments and helping scientists... found this paper. I wrote an Addgene blog post about it and shared the paper with my lab since one of...project. I was invited to participate on a panel about “Social Media for Science Communication” at the ...

...Segmennt of Improved CRISPR Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular weight...of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity...

...protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent...of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting...

... regulating telomere length with TZAP Hot Plasmid 4: CRISPR tools for use in plants.  Listen to the segment...of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology, ...

...Retroviruses and cancer. Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C.S....Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,... be helpful to you. Interested in learning more about a specific virus? We have you covered with guides..., adenovirus, and AAV. If you’re wondering more about what applications these viruses are good for, we...

...iSRIRACCHA) where Cas9 expression is activated using 4-OH-T and doxycycline. A gRNA that targets your desired...found that H2B-GFP expressing cells exhibited a 2-4 fold increase in the number of endogenous indel edits...off target effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA...have been several systems to do this but most are labor-intensive, require multiple fluorescent proteins... Browse all of our CRISPR blog posts Find more about fluorescent proteins Get a refresher on CRISPR with...

...adaptive immune responses, which flies do not have!) (4). The power of Drosophila as a model organism is in...PMID: 22038952. PubMed Central PMCID: PMC3677821. 4. J. R. Huh et al. Digoxin and its derivatives suppress...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome Engineering...

... mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes, such... toxicity in long-term experiments.  Figure 4: The C-terminus of Cre (CreC) and the N-terminus of... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

...populations at single-cell resolution. PLoS Biol. 23(4): e3003048. https://doi.org/10.1371/journal.pbio.3003048...fusion protein using an anti-GFP nanobody (Figure 4). Once incorporated into a condensate, the killswitch...adenovirus particles (Zhang et al., 2025). Figure 4: Schematic of the killswitch-nanobody system. A cell...models, showing that this transfer increases the metabolic and metastatic capacity of cancer cells. This ...macromolecules to serve cellular functions. Along with collaborators, the Denes Hnisz Lab previously discovered that...models By Brian O'Neill The Nadav Ahituv Lab and collaborators have found a way to make adipocytes fight cancer...of fat tissue that is able to waste energy by metabolizing glucose to dissipate heat. Of the genes they...

...and 70186, respectively). Antares Chu, J., et al. (4), likewise, wanted to produce a BRET probe for imaging...modular base-specific gene tagging using a ligase-4-dependent mechanism. Nature Comm. 7, 12338. PubMed...PMID: 26424696. PubMed Central PMCID: PMC4668208. 4. Chu, J., et al. (2016) A bright cyan-excitable orange...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often... emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...

...tubes, and would prevent the pH change upon thaw (Fig.4). To test this, we froze the tubes at -80°C and then...transport or storage on dry ice. Nat Methods. 2013 Apr;10(4):278-9. PubMed PMID: 23538862. 2. Potter M, Lins B...return the sample back to the original pH. What about putting it back in the freezer? We then tested whether...

...under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were first... Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni,... Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic...