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We narrowed to 333 results for: caps

Showing: 61 - 80 of 333 results

  2. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... a system in Streptomyces, a prokaryotic genus capable of producing a variety of natural products. Recently... Schematic representation of cell-free system, capable of producing fluorescent proteins and enzymes. ...Moore et al., 2021.   This TX-TL system is capable of robust expression of high G+C content genes,...relatively simple, controlled “one-pot” reaction capable of reproducing useful natural materials.      ...

  3. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...protein shell known as a capsid, some also have a lipid bilayer surrounding the capsid, called an envelope....particles. As a bonus, this increases the carrying capacity of the viral vector, increasing both safety and..., just to increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce...

  4. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...health science startup requires a great deal more capital to get off the ground, as you will likely need ...your potential contribution to the healthcare landscape.  You will find an extra layer of difficulty launching...projects, but it quickly became apparent that raising capital for such a fund is an impossibility at our early...experts, including Harvard/MIT faculty, venture capital and angel investors, and business development heads...

  5. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ... cargo into a cell. These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs.... Lentiviruses have slightly larger packaging capacities than gamma-retrovirus, coming in around 9 kb....serotypes (12 naturally occurring and more synthetic capsids are routinely being developed) to choose from, ...direct application of virus. However, the cargo capacity of AAV is ~4.7 kb, almost half that of lenti and...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...cells and have an ~8kb packaging capacity. With this packaging capacity, reprogramming factors can be delivered...other delivery methods. Retrovirus has a cloning capacity of ~8 kb, so either a single polycistronic vector...and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral vectors. This ...a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This system is transfected into cells...

  7. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...competitive ELISA use antibodies coating the wells to capture antigen from a sample, whereas antigen is bound...coating the wells of a flat bottomed plate with a capture antibody that is specific to the protein you are...that also recognizes the same target protein. The capture antibody and the detection antibody recognize different...for detecting antigens that are too small to be captured between two antibodies in a sandwich ELISA. While...

  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...proteins are advantageous in today’s genome editing landscape. The need for PAM-less editing   Guides for CRISPR...Walton, et. al, 2020). SpRY uses NRN PAMs and is capable of editing at frequencies comparable to SpCas9....and base editing applications, with high editing capacity in both contexts for NRN PAMs and to a lesser ...other Cas variants described, nearly all genomic landscapes should be editable with CRISPR technology.   ...

  9. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...mutated to inactivate the protein’s nucleolytic capability, and this nuclease-null or “dead” Cas9 (dCas9...complementarity to a target site, Cas9’s DNA binding capability remains intact while its nucleolytic activity... RNA hairpin, such as the MS2 hairpin, that is capable of recruiting additional effector domains (4). ...with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator, robust...

  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...FP-fusion to rhodopsin resulted in electrochromic FRET capable GEVIs, with rhodopsin quenching the added fluor...available with red-shifted fluorophores (find FRET capable GEVIs here!). The only issue with these GEVIs? ...sensing component and a self-labeling domain that is capable of binding a Janelia Fluor (JF). The voltage-dependent...pseudotyping is also a powerful tool that swaps capsid proteins from different viruses to optimize them...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...different packaging capacities depending on the virus. AAV has a packaging capacity of ~4.7 kb, while lentivirus... lentivirus has a capacity of 8–10 kb. Time Viral production in mammalian cells is relatively quick. After...transfer plasmids have a much larger packaging capacity — up to 38 kb! Generally, insect cell production...

  12. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...genes; and the pRepCap plasmid containing replication factors and serotype-specific capsid genes.  In this...genome-containing (full) and non-genome-containing (empty) capsids, thus leading to an overestimate of transducible...1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production...

  13. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...affinity purification in situ of regulatory elements (CAPTURE) (Liu et al., 2017). This system utilizes dCas9...through proteomics and the chromosome conformation capture (3C) technique. In addition to 3xFLAG and biotin...when inappropriately expressed can cause facioscapulohumeral muscular dystrophy (FSHD), a disease for ...Central PMCID: PMC6286148. Liu, Xin, et al. "In situ capture of chromatin interactions by biotinylated dCas9...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...but only transiently. However new viral vectors capable of providing long-term gene expression in retinal...and severe visual impairment that faithfully recapitulated the human condition. Luck brought all these ...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...further improve the technology: engineering of new capsid variants, designing expression cassettes to regulate...

  15. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...binding capacity and high protein retention. It comes in a variety of pore sizes and protein capacity, allowing...are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than... is a little quicker to use, with high binding capacity, though it has lower retention after binding and...

  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...look specifically at the characteristics and capabilities an ELN needs to facilitate true reproducibility...without access to them, the record of research captured in the ELN is incomplete. Thus, to support reproducibility...for discussion and review. It’s also possible to capture Slack conversations and import them into RSpace...and workflows that went into it. The ability to capture the conversations and associate them with a particular...

  17. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    .... If you missed it, here’s the recap! The twelve days of CRISPR recap On the first day of CRISPR, Addgene...day of CRISPR, Addgene gave to me: a method to capture promoter-enhancer 3D chromatin structures using...

  18. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...Transfer Plasmid : 1 pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type of tissue...using 28 μg of total plasmid (Transfer, helper, and RepCap added together) per 15 cm dish or 2500 μg of plasmids...

  19. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the post-translational modifications...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...
Showing: 61 - 80 of 333 results