We narrowed to 152 results for: cycs
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TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....grow and maintain in the lab, have a short life cycle of 8 to 14 days, and produce a large number of offspring...small, transparent worms that have a rapid life cycle and large brood sizes. The majority of C. elegans...
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Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....the most common approaches is to use sequential cycles of primary and secondary antibody labeling and ...secondary, image again, inactivate, and repeat these cycles for all your targets. Of course, you’ll need to... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc. are available... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course, one cannot truly predict the perfect...stable than other viruses, multiple freeze thaw cycles can still reduce AAV titer. For long term storage... -
28 Hot Plasmid Technologies from 2015
TypeBlog PostPublishedDec. 23, 2015, 3:30 p.m....endosomal trafficking to signaling pathways to the cell cycle. Good tools for measuring intracellular pH are required...movement of two or more targeted loci during the cell cycle or cell differentiation. This tool should delight...visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem cells and a series... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...presence of the inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the... -
22 Hot Plasmid Technologies from 2014
TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m....and rtTA. The cells were pulsed with 2µg/ml of Doxycycline for 2 hours, and imaged on the Keyence BZ-9000...dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation. Chemical... -
New FLIS Plasmid (April Fools 2022)
TypeBlog PostPublishedApril 1, 2022, 1:15 p.m.... genetics research, for decades. Its short life cycle, reasonably close genetic homology to humans, and... -
#Deckthelab Contest Winners 2017
TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m....challenge for her lab mates to create the best-recycled lab supply ornament ever! & they totally succeed... -
New Viral Vectors - Summer 2024
TypeBlog PostPublishedJuly 9, 2024, 1:15 p.m....ubiquitous and constitutive promoter. Use this for doxycycline inducible (tet-off) gene expression! We've also... -
2018 #DeckTheLab Contest Winners
TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....nosed reindeer from @ladiesinlabcoats Many teams recycled lab materials into holiday decorations. We appreciated... -
What's New in CRISPR - Winter 2018
TypeBlog PostPublishedDec. 18, 2018, 1:17 p.m....transcription factors with GFP. As part of the Encyclopedia of DNA Elements (ENCODE), a consortium aimed... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog PostPublishedNov. 10, 2015, 3:30 p.m....Georgieva et al. created tagged versions of the cyclin Cdc28 and the chaperone Ydj1, both of which display... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....which get eaten by another snail, completing its lifecycle. Halloween genes The Halloween genes are a set... -
Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m....on black screens, but not to know even the life cycle of the creature they deal with.” So photography ... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m....libraries include enrichment against kinases, cell cycle proteins, and more. “In the past, genome engineering... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m....work, see how many pictures we take to make a walk cycle of one step. Challenges in the LEGO science video... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase is proving an... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....ColE1 A Relaxed pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent...