...was certain I would not end up in a life sciences career but life has a way of making unexpected decisions...what it was like to work at Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke...

...that deserve a second look. 5 Great Apps for Lab Life This very useful post was contributed by guest blogger...growth medium, these apps can help make your lab life a bit easier. Since the post doesn’t provide an ... lab life...

...past few years, including an article in the BBC Wildlife Magazine. It is easy to see how these incredible...information on black screens, but not to know even the life cycle of the creature they deal with.” So photography...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert...

...bold. You don’t have to have all of the listed qualifications to apply for a position.  A job description...application). Beware the myths of scientist “overqualification” but it may not be productive to apply for...resumes The cover letter must address how you are qualified and why you are interested specifically in the...

..., allowing me to pinpoint areas of my career and life where I excel (and thus feel grateful) and identify...once said: “There are only two ways to live your life. One is as though nothing is a miracle. The other...quote encourages us to contemplate our approach to life and how our viewpoints influence our encounters....

...articles openly available on PubMed Central (also eLife, PLOS and Figshare). Some use cases include: Cataloging... collections from a large section of open access life sciences data. You can use ReFigure to coherently...we can only connect figures from PubMed Central, eLife, PLOS and Figshare but we are working on expanding...

...researcher on parasitoid wasps; and an essay on the life of Srinivasa Ramanujan, to be precise) and remarking...neurological health risk. Having played the sport my entire life, I was curious as to what the research had to say...chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry. A research...

...multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...created by James Trimmer, PhD, of the University of California Davis School of Medicine. This deposit includes...

...which limits its use for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...

...with and/or requests materials from Addgene can qualify. We also reward improvement against one’s own record...Hospital  Brandeis University  Broad Institute  California Institute of Technology Cold Spring Harbor Laboratory...University of British Columbia  University of California, Irvine University of Massachusetts, Worcester...

...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily...

...Individual Development Plan (IDP) and uses it to match qualified scientists to jobs in industry, academia, and ...find open positions and view their required qualifications and skill sets Taking the time to identify...Communication Networking As the number of qualified scientists entering the workforce increases, EAs...

...to target cells as a pooled library, which will amplify each TF to determine how its presence influences...processes. For help with library transformation and amplification, refer to the Zhang lab’s previously published...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library...

...multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi: 10.7554/eLife.43322. PMID: 30667360; PMCID...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...

...evidence (in psychology, cancer biology, pre-clinical life science work, economics) that published research... their study plans at a point in the research lifecycle when suggestions can be incorporated into the ...and as the Director of Advising in the Division of Life Sciences at Rutgers University. His dissertation...

...with the desired mutation, then use those to PCR amplify the template plasmid. The parental, nonmutated ...primers, each containing the desired mutations, to amplify both strands of a plasmid template. In addition...pitfall of REPLACR-mutagenesis is that you must PCR amplify the whole plasmid, which could introduce mutations...

...allowing the device to take on a community-driven life of its own. Nevertheless, there are still many open...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and... B., Marsikova, B. et al. Open microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 ...

...plasmid (Option 1) or added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning...is to "domesticate" your fragment: PCR-based amplification can be used to create silent point mutations...high-efficiency step. References: Sequential amplification of cloned DNA as tandem multimers using class-IIS...

...strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent...directed toward AHA. Now the rolling circle amplification could only take place if the two antibodies ...

...antibody, a primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can...ELISA, the only difference being the additional amplification step, which helps to visualize low-abundance...DNA damage and instability detection. Cell. Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007...