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We narrowed to 91 results for: myc

Showing: 61 - 80 of 91 results

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pSiM24: Simplifying Plant Genetic Engineering

Type

Blog Post

Published

Sept. 29, 2015, 2:30 p.m.

...functional in a variety of plant species. The kanamycin antibiotic resistance gene is controlled by a ...
What's New in CRISPR - March 2020

Type

Blog Post

Published

March 10, 2020, 1:15 p.m.

...that controls repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the...
Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Type

Blog Post

Published

Oct. 29, 2015, 2:30 p.m.

...resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected...
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

Type

Blog Post

Published

April 7, 2015, 4:21 p.m.

... various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily...
What the HEK?

Type

Blog Post

Published

April 18, 2023, 1:15 p.m.

...cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also particularly ...
Four Factors that Differentiate the Stem Cell Field

Type

Blog Post

Published

Sept. 28, 2016, 2:35 p.m.

...by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors). As the Yamanaka lab's...
Visualizing Translation at the Single Molecule Level

Type

Blog Post

Published

Aug. 1, 2017, 1:15 p.m.

...Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is ...
K. phaffii: Rising to the Occasion

Type

Blog Post

Published

April 4, 2023, 1 p.m.

...Resources References Liu, Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Type

Blog Post

Published

July 28, 2015, 2:30 p.m.

.... It also gives cells antibiotic resistance to neomycin so that you can select the population of cells...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

Published

April 16, 2015, 3:45 p.m.

...End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128...
Plasmids 101: Cre-lox

Type

Blog Post

Published

Jan. 13, 2015, 3:47 p.m.

...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Type

Blog Post

Published

May 11, 2021, 1:15 p.m.

...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method...
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Type

Blog Post

Published

April 4, 2019, 12:53 p.m.

...the human gut bacteria B. thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

Published

April 23, 2018, 2 p.m.

...targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

Published

March 23, 2021, 1:15 p.m.

...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

Published

Dec. 14, 2021, 2:15 p.m.

...lentiviral backbones expressing blasticidin or puromycin resistance genes. It’s that simple! Good luck...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

Published

March 28, 2018, 6:52 p.m.

...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

Published

Oct. 22, 2015, 2:30 p.m.

...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

Published

Oct. 6, 2015, 2:30 p.m.

...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

Published

Aug. 27, 2020, 1:15 p.m.

...Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org...

Showing: 61 - 80 of 91 results

Results per page:

pSiM24: Simplifying Plant Genetic Engineering

What's New in CRISPR - March 2020

Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

What the HEK?

Four Factors that Differentiate the Stem Cell Field

Visualizing Translation at the Single Molecule Level

K. phaffii: Rising to the Occasion

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

CRISPR 101: Non-Homologous End Joining

Plasmids 101: Cre-lox

Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Hot Plasmids and Viral Preps - March 2021

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Tips for Screening with Yeast Two Hybrid Systems

Tips from the Repository Trenches: Using Barcodes to Track Samples

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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