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We narrowed to 336 results for: nam

Showing: 61 - 80 of 336 results

  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...controls are required to make this method useful for dynamic experiments in which FRET changes are large. Two..., but it is destructive and cannot be used for dynamic measurements. Extra care should be taken so as ...donor is shortened, allowing FLIM to give an unambiguous value of FRET efficiency. As you don’t measure...

  2. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant was made...PMC4927352 7. Hink, Mark A., et al. "Structural Dynamics of Green Fluorescent Protein Fused with a Single...PubMed PMID: 18155467. 13. Chen, Baohui, et al. "Dynamic imaging of genomic loci in living human cells by...

  3. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...populations over time. The ability to track single cell dynamics at the organism level has been made possible by... the cerebellum of mice, the team appropriately named their technology Brainbow. Brainbow 1.0 used the...multicolor cell labeling systems allow single cell dynamics in a variety of organisms to be observed in more...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...MTRIAOT enabled real-time observations of oxytocin dynamics in live mice under a variety of behaviors, opening...real-time measurement of extracellular oxytocin dynamics in the brain. Nat Methods. 2022 Sep 22. pii: 10.1038...means operating at the outer edge of the sensor’s dynamic range. With this in mind, Ed Boyden and colleagues...

  5. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...Ethan Hawke, Uma Thurman and Jude Law. The movie’s name is itself a combination of the four bases of DNA...involves Langdon chasing an anti-villain geneticist named Zobrist who designs a virus that can potentially...Kartik’s research focuses on the use of molecular dynamics simulations and machine learning to develop a ...

  6. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...particularly beneficial when studying dynamic processes like mitochondrial dynamics. This process, involving mitochondrial... and imaging techniques to study mitochondrial dynamics in mammalian cells. Biophysics Reports, 3(4–6)...

  7. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier name CGBE1, for “C → G Base Editor 1,” and had a C →...clear why this is, but it might point to a more dynamic picture of chromatin than an accessible/inaccessible...track of which acronym is which. Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA ...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Vivid (VVD, N. crassa) (Kawano et al., 2015). Nicknamed paCas9-1 and consisting of the fusion proteins...photoactivatable CRISPR strategies Publication System nickname Photoactivatable moiety Cas9 variatn CRISPR protein...Phillips-Cremins JE (2019) LADL: light-activated dynamic looping for endogenous gene expression control....

  9. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...Suggest a Plasmid" form Fill out the form with the name of the specific plasmids you’d like to suggest and...at us! Tweet @Addgene with #suggestaplasmid, the name of the plasmid and a publication where we can find...

  11. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...brain tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt.... All of this requires genes to be regulated dynamically.” To understand how that works in memory formation...

  12. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ... same time, it sucks out its contents (hence the name Vampirovibrio). What’s left of the algae cell is...exoskeleton formations. These genes were appropriately named as spook, spookier, phantom, disembodied, shadow...

  14. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... they allow one to monitor compartment/protein dynamics or evaluate protein turnover rate. In many cases...photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available GFP lines. Like...

  15. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...data includes reagent type, target species, target name, host organism, isotype, clone details, vendor, ...Everyone who contributes to the IBEX Knowledge-Base is named on the Zenodo release in keeping with their value...

  16. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...generation of norepinephrine sensors was developed, named GRABNE, which helped researchers visualize norepinephrine...generating variants with improved affinity and dynamic range of both nLightG and nLightR, and may soon...

  17. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...genome. The Sleeping Beauty transposase gains its name from the fact that it was recreated from a defective...without the use of viral vectors. In 2009, it was named Molecule of the Year. Additionally, in 2014 researchers...

  19. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...combines scRNA-seq and lineage tracking to track the dynamics of gene expression and clonal history associated...fluorescent proteins help researchers study single cell dynamics in model organisms Resources on Addgene.org ...

  20. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...targeted to lysosomes for degradation. Autophagy is a dynamic process which involves autophagosome synthesis,...however, has inherent limitations because it turns a dynamic process into a static measurement and it provides...
Showing: 61 - 80 of 336 results