Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
Type
Blog Post
Published
Sept. 13, 2018, 1:55 p.m.
...guides are used to call hits from the growth screen, p-values were less significant than when non-targeting...non-targeting guides are used as controls. Less significant p-values lead to more false-negatives results (i.e. ... library resulted in both increased and decreased p-values of known ricin regulation genes compared to...