We narrowed to 196 results for: p
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TypeBlog PostPublishedMay 25, 2021, 2:29 p.m.... J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018...
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Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....and resources References Banasik, M. B., & McCray, P. B., Jr (2010). Integrase-defective lentiviral vectors...Bayford, J., Brown, L., Quaye, M., Kinnon, C., Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar,... Central PMCID: PMC189872 Schröder, A. R., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman, F.... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m.... PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules...Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck,.... 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated Cell Sorting Using Targeted... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m.... for the wild-type. However, variants such as the P.1 (Gamma variant) and the B.1.351 (Beta variant) were...lowest levels of cross-neutralization occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....hypothesis testing statistical tools that generate a p-value. As an example, if you hypothesize that growth...significant result that may be the result of noise, p-hacking, or unclear methods. Many thanks to our guest... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation... https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl, R., & Cooley, R. (2022). Site-specific Incorporation... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....References and resources References Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System...www.ncbi.nlm.nih.gov/books/NBK27106/ Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....stays open for a longer duration upon activation. p-mcherry-C1-iC++_CA - A slow cycling (open for minutes...20026026. PubMed Central PMCID: PMC2823934. Pai, Vaibhav P., et al. "Transmembrane voltage potential controls... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m.... 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein instability following... B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M, Cleaver BD, Clément N, Byrne ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....organism. Precursor, or pre-tRNAs are cleaved by RNase P and Z in eukaryotes (E in bacteria), and only a few...cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. doi: ... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....s41467-019-12339-7 Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, Klasić M, Zoldoš V (2016) Repurposing...Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing in vivo by programmable... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon... effectively able to introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens...doi.org/10.1038/nbt.3043 Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...10.1038/nbt.2508 Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington...10.1128/mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m..... https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987) Single-Step Method of RNA Isolation.../doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....genes that affect autophagy (ATG) in yeast. Atg8p (“p” stands for protein) associates with autophagosomal...PMC2852113. 4. Klionsky, D. J., Cuervo, A. M., & Seglen, P. O. (2007). Methods for Monitoring Autophagy from ... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m....Horwitz, N., Beggs, A. H., Wagers, A. J., & Sabeti, P. C. (2021). Directed evolution of a family of AAV ..., Papworth, G., Watkins, S., Trucco, M., Robbins, P. D., Li, J., & Xiao, X. (2006). Widespread and Stable... Emily P. Bentley... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m.... J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m.... immunity to viral invasion. His lab is studying P. furiosus, a hyperthermophile that exists in sea vents...regions of the prokaryotic genome. Then he desribed how P. furiosus has systems to defend against both DNA and... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog PostPublishedOct. 17, 2024, 1:15 p.m.... Emily P. Bentley... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine...383. PubMed PMID: 12605205. 13. Yan, Qi, and Marcel P. Bruchez. "Advances in chemical labeling of proteins... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m.... plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K.....), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011...