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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 231 results for: pET

Showing: 61 - 80 of 231 results

  1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ... to poor lab techniques. These include reusing pipettes for multiple cell lines rather than using disposable...aerosols that are generated (while talking or pipetting) during working in the laminar airflow are also... are tightly packed with seals. Use disposable pipettes to avoid cross-contamination. Make sure you keep...

  2. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...done in reverse. Assuming you want to become competitive for a Group Leader position, what will you need... you get more publications, you will be more competitive for fellowships. If you explore your system more...job search. Therefore, you must balance these competing goals. Knowing when to follow a lead and when ...

  4. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came together and delivered very ...

  5. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave...species for the challenge, but one species always outcompeted the other species.  When he found the chromoprotein...

  6. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular, generating ...Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter...

  7. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...dire:  The current system is unsustainable, hypercompetition is corroding values, we are shifting away ...such as the National Postdoc Association Core Competencies and the Science Careers’ myIDP). Dr. Galit Lahav...

  8. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic tool that allows for the labeling...PhiC31 irreversibly recombines one of the two competing attP sites to drive expression of either a stop...

  10. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... Fan, recently shared our protocol for making competent cells to save time and money. My other stingy ...this apparatus for 15 years before I hung up my pipets.   ...

  11. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...overnight. 4. Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol ...transforming your reaction into chemically or electro-competent E. coli - excess salt causes arcing during electroporation...

  12. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...filing cabinets, a rickety old incubator, a few pipettes, and some home-made equipment. It was humble in...year with a computer science degree and had zero pipetting experience. However, after a few months of dedicated...

  13. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...during transformation First, use enough DNA and competent bacteria to ensure all components of the library...library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications...

  15. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...of armor to protect their host from predation (Petroni et al 2000). With a better understanding of how... Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & ...

  16. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...guide for the quest of a perfect "fit". How competitive is the lab? Some labs have only one author plus...labs around the world. You may prefer a more competitive style to spur you to succeed or you might enjoy...

  17. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...help manage details. For example, setting up your pipette tips to match your plate layout can help you track...too - one of my coworkers would build a wall of pipette tip boxes when she particularly needed to focus...

  18. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...-mutagenesis also requires making your own electrocompetent cells in which the phage recombination machinery...Purified Enzymes Required No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis...

  19. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...(anthocyanins), and the plant circadian clock. Petunias have been a model organism for plant research ...mechanisms and components of the circadian rhythm in petunias. Because of the extensive research that has come...
Showing: 61 - 80 of 231 results