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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 105 results for: pam

Showing: 61 - 80 of 105 results

  2. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...CRISPR cannot be used (e.g. there are no suitable PAM sequences nearby or your gene of interest is difficult...

  3. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene The Human GlycoGene...

  4. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... scan along the double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type Cas9 and a gRNA...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...variants to develop five new base editors with distinct PAM sequences, expanding the number of available target...and confirmed that the fusion protein retained the PAM properties of the individual Cas9. Kim et al. also...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast...

  8. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...non-dividing cells. Cas13 enzymes also don’t require a PAM sequence at the target locus, making them more flexible...

  11. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...site-directed mutagenesis can be used to mutate the PAM sequence (an NGG sequence critical for Cas9 cleavage...

  15. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...stricter sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al. 2013). This...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Within the editing window -50 to +50 bp from the PAM site, CRISPR-X produces point mutations at a rate...

  20. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...study alpha2-AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is... increased selectivity for norepinephrine over dopamine. All three sensors, however, did display some ...some reaction to high concentrations of dopamine. This cross reactivity will need to be accounted for in ...
Showing: 61 - 80 of 105 results