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...elements for vector propagation. This post, along with a future companion post on mammalian vectors, will catch...In our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance...of plants and animals. Unlike bacteria, yeast can post-translationally modify proteins yet they still share...Schizosaccharomyces pombe, vectors at the end.) In a previous post, we briefly discussed how the regulation of bacterial...

...biology blog posts Find tips for Arabidopsis transformation Read our Plasmids 101 blog posts Resources ...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with...transient technique that uses a virus and the plant's post-transcriptional machinery to silence genes of interest...MoClo system. The vectors mentioned in this blog post above are ready to use vectors, but with the MoClo...and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene silencing method." International...

...CRISPR blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts Resources...seem endless - and overwhelming. In the first blog post in this series, I’ll highlight terminology you should...mouse seems less overwhelming! In the second blog post of this series, I’ll discuss crossing and maintaining...

...connecting. You can see what they post and occasionally comment on their posts as appropriate, possibly giving...companies and organizations to see their news and job postings. 4) Use LinkedIn to pay it forward The best way...profiles. 7) Keep it professional at all times Do not post or comment on anything that is not relevant to business...for using social media as a scientists Read blog posts about networking ...will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile” instead of “...

...In this post of the Careers in Science Communication blog series, you’ll hear more about Caitlin Runne-Janczy...how Caitlin got into scicomm and landed her first post-grad school job, head over to part I of the interview... proposals, a few PhD theses, and occasionally posting something science-related on Facebook. “I think...Channel of the blog so you don’t miss the future posts in this series! Additional Resources on the Addgene...

...lower the probability of off-target editing. In this post, we’ll summarize how IDT (Integrated DNA Technologies...nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the RuvC domain, ...donors (if possible) The text and images in this post were adapted from an IDT webinar: Optimized methods...Mollie Schubert and Shuqi Yan for reviewing this blog post. Additional Resources on the Addgene Blog Learn...

...tools not listed in today’s post? Share them in the comments below. This post was written in April 2020 ...virtual molecular biology toolbox organized, today’s post features a list of free online molecular biology...functional information, such as expression patterns, post-translational modifications and polymorphisms Codon...

...about what makes a good mentor, see the previous post in this series. Check out Joanne's Reddit AMA Different...provided a downloadable PDF at the end of this blog post. This document includes a list of questions to ask...Academic Mentors by @drmellivora, an excellent blog post about workplace bullying featured on the always ...developing your career in science.  Read other posts in our Science Mentors series: Part 1: "What Makes...shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose will be ...Different types of mentors for grad school vs. postdoc Choosing an advisor to complete a successful PhD...PhD is different from choosing a lab for postdoctoral work. However, selecting a lab with a PI who is ...

...creative uses of old posters, pipette tip boxes, and more (stay tuned for a few blog posts coming up about ...Eppi the red nosed reindeer.       View this post on Instagram     Eppi the red nosed Reindeer! ...research #cardiovascularresearch #parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats...

...scicomm ♬ original sound - Addgene We'll be posting these animations regularly from now until May, ...follow us to get updates whenever a new video is posted! If TikTok isn't your platform, all of our videos...

...active under specific circumstances. In a previous post, we discussed inducible promoters, which can be ...either category, but those are a subject for a future post! How are repressible promoters regulated? Like...as the inducible manner detailed in our previous post. In the past 30 years since the original Tet-Off...as the inducible manner detailed in our previous post. While originally developed for use in mammalian... modular vector for ease of use! Conclusion This post contains only a brief overview of some of the most...The repressible promoter systems detailed in this post can also be combined with other systems, such as... what might work best for your experiments! This post was originally written by Mary Gearing in 2018, ...

... For my other posts on lab sustainability, check out my Recycling and Innovations posts. Making small ...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...

... Research Toolbox blog posts on free tools for making scientific graphics post and social media for scientists...going on in other areas of science. Scientists often post tweetorials, or threaded summaries, of their papers...topics.  Twitter paper bots: Twitter bots tweet posts that contain specific keywords. It’s sort of like...covered four ways to find relevant papers in the post, there are many more ways out there. Let us know...

...Last summer, we posted about our bioinformatics quest, which led us to Seqera Labs, a company that provides...infrastructure, and secure collaboration.  In that post, we talked about Addgene’s data processing needs...

...Other Career Blog Posts Read the Networking for Scientists Series Read our Blog Post about Choosing a ...This post was contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, ...expectations of the program and their baseline skills and a post-program self-assessment, participants completed ... results. Participants’ responses to the mid and post-program self-assessment revealed that their knowledge... degeneration. She is also a blogger who writes posts for the blogs of MasScience, ECUSA, IMFAHE and Bostonadas...linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where...

...research. When you're done with this post, check out our follow up post on repressible promoters. How are...only active under specific circumstances. In this post, we’ll discuss inducible promoters, which can be...Two-Component Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org...

...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering...Bioengineering. Read their previous blog post about how they started their company here. The first transgenic plant...Biophysics from UC Davis in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University...Like RevBio on Facebook Read more Addgene blog posts about synthetic biology ...

...discernable effect on mammalian cells. In this blog post we will discuss how mammalian plasmids differ from...covering this ever-versatile fluorophore in a later post, so stay tuned! Transient transfection and the elusive...plasmid DNA into the genome. For the purposes of this post, we will define positive selection as the means ...should not be added to culture media until 24-48 h post transfection when creating stable cell lines. ...

...gene enables selection of cells with the plasmid post transfection. The 2A "self cleaving" peptide causes...This tag can be used to test for SpCas9 expression post transfection by western blot. Overall, this is a...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...

...gap using gifs”: https://amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-some-enzymes-for...Science communication check out these SciComm blog posts. References 1. Bourdeau, Raymond W., et al. "Acoustic...PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering...