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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 219 results for: sms

Showing: 61 - 80 of 219 results

  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...

  3. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...Disable the other repair mechanisms Mammalian cells have 2 primary repair mechanisms besides HR - Non-homologous...

  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...have different levels of cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example...differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to the cells ...

  5. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome...cells to understand the genetic and biochemical mechanisms behind their distinct phenotypes and to design...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...

  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...indispensable tools for manipulating cells and organisms. They can be used for a variety of research purposes...numerous variants have been developed with different mechanisms to inactivate the integrase.  The IDLV life cycle...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...

  12. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...He currently works to understand pathological mechanisms of Alzheimer’s disease by developing novel iPSC-based...

  13. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...expression. (a) Several engineered capsids with unique tropisms are now available. These include variants with...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species..., 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated in parentheses...

  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...genomes. A) Components of the INTEGRATE system. B) Mechanisms of RNA-guided transposition with INTEGRATE. The... Transposon Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...known is that different serotypes have different tropisms but, as you can see, AAVs of each serotype can...Adeno-associated virus serotypes 1, 8, and 9 share conserved mechanisms for anterograde and retrograde axonal transport...

  17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ...

  20. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... Australia.    🤩 our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX...
Showing: 61 - 80 of 219 results