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Showing: 61 - 80 of 296 results
  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...recessive gene. Ma et al. claim that CRISPR would still be helpful in increasing viable embryos, especially... in terms of editing success rate. Would CRISPR still be worthwhile for a given disease if it increased...
  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...make sure yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather...challenges, your interviewers might.  You’ll probably still be required to give a job talk for a remote interview...
  3. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...move in the right direction, the increase notably still does not meet the suggested postdoc salary ($50,...advocating for change: from developing a strategy to distilling a message for the appropriate audience and communicating...
  4. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...how close is close enough for the desired edit to still occur if the cut site is sub-optimal? As with everything...Nonetheless, in an AT-rich region, these Cas9s may still leave you choosing between efficiency and location...
  5. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ... cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook from time to...worked weekends and evenings on the project, but still continued to generate new ideas as they popped into...
  6. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...and monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies...EQKLISEEDL) - larger than the substance P peptide, but still quite small. However, unlike the substance P tag...
  7. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... related to the actual data collection task but still be beneficial to both.  Coming up with creative ...with the company so they know I’m excited while still being able to take my vacation? I want to learn ...
  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...fluorescence all have room for improvement. These sensors still have many useful applications, though, and if you...this two-part fluorescent system, and rhodopsin still functioning as the voltage sensor. FP-rhodopsin ...
  9. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...
  10. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...and well-documented history of racial bias, which still impacts and influences current research. Understanding...five-team response to address this issue, but it still points to a far larger and more complex problem ...
  11. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...too old to transform. This transformation could still work, but the efficiency will be low. Tips: Use...plants: If you transform early, your plants will still have plenty of time to produce seeds after you expose...
  12. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...period of time. Though climate change certainly still is and will continue to be a “critical consequence...current period of rapid flux, the chapter might still feel slightly dated by next week.   A stepping stone...
  13. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions...instance, the DBD of the fusion protein shown is still able to bind to the upstream activation sequence...
  14. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...regeneration, trace amounts of DNA (3 femtograms/ul) could still be eluted from the column, as detected by qPCR, ...break-up with your DNA purification kit, you can still reduce the number of spin columns you need to complete...
  15. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...its first steps! However, retroviral vectors can still direct the translation of proteins because they ...and transduction by vectors (which we sometimes still call “infection” as a shorthand, although it is ...
  16. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...technique is still used today to test for HeLa contamination. HeLa contamination is still a problem today...
Showing: 61 - 80 of 296 results