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We narrowed to 242 results for: virus

Showing: 61 - 80 of 242 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...mutagenesis in humans (1) and AAVs are non-enveloped viruses, so they are resistant to alcohol-based disinfectants...freezing and thawing. Although more stable than other viruses, multiple freeze thaw cycles can still reduce AAV... structure (scAAV) (7). As single stranded DNA viruses, AAVs normally require second strand synthesis ...phenotype. Previous neural tracing methods used viruses that were potentially toxic and would result in...4. Castle, Michael J., et al. "Adeno-associated virus serotypes 1, 8, and 9 share conserved mechanisms...Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently...efficient transduction by recombinant adeno-associated virus vectors." Journal of virology 70.5 (1996): 3227-...

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... to just dive right in and start injecting your virus, a successful AAV experiment starts with validation...compositions of AAV capsids, which determine how a virus enters the cell. Different serotypes utilize a number... serotypes have broad tropism (the ability of a virus to enter a particular cell type), but there are ...begin testing, you’ll next need to decide how much virus to put into your system, which is controlled through...picked, since that can affect the spread of the virus.  A better way to define high titer, then, is this...recombinase-dependent vectors) increases with the amount of virus you add, so it is strongly recommended to optimize...(2018). An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal...

  3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...poorly understood,” says Qiu.   Qiu developed a dual virus system using both optogenetics and chemogenetics...neurons is in progress, she has validated the dual virus method to study CR neurons which are depicted here...Schematic diagram of the construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate... response due to blue light stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses...) Immunohistochemistry verification of the dual virus expression in CR neurons of the spinal cord. Created...

  4. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid which contains...Cre-Lox system.  WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE...Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...the Inverted Terminal Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https:/...

  5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...engineering viruses Acr genes can be used as positive selectable markers in engineering viruses. For example...Editing of Sulfolobus islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695...difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz et al., 2018). Only viral particles...Papayannopoulou T, Lieber A (2018) HDAd5/35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene...single stranded DNA genome of AAV in the native virus. Since the WT sequence is relatively unstable, a... L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific Inverted Terminal Repeat Sequence...the Inverted Terminal Repeats of Adeno-Associated Virus. Hum Gene Ther Methods, 30(6), 206–213. https://...Repeat Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5...

  10. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically, viruses are able...certain forms of gene therapy - avoiding the use of viruses is also important for social and regulatory reasons...

  11. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ... serve the scientific community. Here are a few virus-related protocol videos that may help to save you...produce vital tools in the lab such as antibodies or viruses. If you’re new to tissue culture, check out this...

  12. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes and binds to ... distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK could...including clinical diagnostics (e.g. pathogen or virus detection), therapeutics and sensitive genotyping...specific DNA sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently...Learn about using toehold switches to detect Zika virus   Learn more about targeting RNA with Cas13a (C2c2...

  13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... human lung cancer cell line), armed only with viruses obtained through Addgene’s viral service and the...repeatability and bad news for a screen. Because Addgene viruses are provided at a specific titer, you can better...expression was the most efficient for gene editing. A virus encoding GFP plus a gRNA targeting GFP (pXPR_011...activity in my stable A549 cell pools. Using this virus, it’s expected that Cas9 activity, guided by the... I just randomly chose one of my stable pools. Viruses containing BRAF gRNA (BRDN0000561167 and BRDN0001146266...regions of your gene. I used existing BRAF gRNA viruses, but you can find a list of validated gRNA plasmids...Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...

  14. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...the purification step because they are inside the virus itself. While these impurities are generally considered...Prokaryotic Sequences during Recombinant Adeno-Associated Virus Production and Their in Vivo Persistence after Vector... in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing. Molecular Therapy - ...Control Testing of Recombinant Adeno-Associated Viruses. In: Methods in Molecular Biology. Humana Press....org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus Browse Viral Plasmids ...

  16. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...adaptive defense strategy against life-threatening viruses. Evolving to survive Although the CRISPR sequences...ticket to ensure prokaryotic survival; however, viruses, too, are famous for their ability to devise new...strategies to reproduce within a host cell. A few virus particles have learned to circumvent the CRISPR/...

  17. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The vectors could integrate...Resources on Addgene.org Check out Our Lentivirus Guide Learn about Virus Biosafety Find Lentiviral Vectors...possibility of creating a replication-competent virus via recombination between the transfer and packaging...events necessary to create a replication-competent virus. Second, Tat is eliminated through the addition ...useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability...dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, but without... Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to infect non-dividing cells...

  18. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...labelled with an AAV1 Cre virus combined with a Cre-dependent AAV9 tdTomato virus in the mPFC.  The AAV9 ...

  19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...brain, researchers inject genetically modified viruses encoding microbial rhodopsins into specific cerebral...and Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become increasingly popular for gene...cells? This can be achieved using Cre-dependent viruses, such as the optogenetic FLEx vectors in combination...cell-type promoter. Once injected into the brain, the viruses infect all the cells with an inactive opsin gene...References Abdallah, Khaled, et al. "Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary...

  20. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...working with AAV or lentivirus in your research, you might want to use one of our virus backgrounds. Addgene...barcoding libraries, and more. Download the green virus background.           Maybe your viral research...well as transfer plasmids. Download the orange lentivirus background.         We also have ready-to-use...background for you, too! And we have a COVID-19 and coronavirus collection page, where you can find plasmid tools...
Showing: 61 - 80 of 242 results