We narrowed to 96 results for: yeast
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TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....genomes. Unlike plasmids used for expression in yeast and bacteria, plasmids used for stable transfections...
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Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....Gate Assembly. They built this toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....brightness can be made. This strategy has been used in yeast by Lee et al (2013) and in nematodes by El Mouridi... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m.... for use in model organisms such as E. coli and yeast. Because this approach uses standardized parts, ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....techniques being developed. In the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....assayable with CRISPR. Another method, popularized in yeast, are temperature sensitive screens. These involve... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....Run) and to detect protein-protein interactions (yeast-two hybrid, Co-IP, BioID), and that’s just to name... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....strategies are not available to those working with yeast or plants. Using MoClo, or better yet, a pre-made... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous recombination... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m..... Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion of... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....efficiently and reversibly bias inheritance in wild yeast. bioRxiv 013896 (2015). doi:10.1101/013896. bioRxiv...methods are easy to implement and have been tested in yeast5. Ecological confinement involves performing experiments... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m.... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m....comparison to other display systems using phage and yeast (109-1010 members). This selection step involves... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....It does work in mammalian cells, mice, and even yeast, though (we have a kit!). Choosing a degron tag ... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....specific secondary structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m....organisms for expression in prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....open-reading frame (ORF) libraries have been generated for yeast (Kim et al., 2019) and fruit flies (Bischof et al... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....available for many expression systems: bacterial, yeast, and mammalian. These plasmids contain both untagged...