We narrowed to 842 results for: Eras

...circuits and dynamics. FPs are generally bright, stable and well tolerated by cells. They can be used for...neither disturb POI folding nor its ability to interact with protein partners within the cell. However...can affect protein folding and protein-protein interactions. Also, overexpression of FPs can trigger protein...ideal probe should combine the solubility, cell tolerance, and optional fluorescence of FPs with facile ...monster” fluorescent proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags...

...genotypic and phenotypic artefacts. The life science literature is a litany of false interpretation in cell culture...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...out by the commercial repository. Repositories generally ensure cell line identity and purity making it...to the original as possible and so any results generated are reproducible and reliable. When you order ...which can be stored in liquid nitrogen long term, preferably some of these vials will be stored in a separate...identity. The Nature Publishing Group recently reiterated its strict stance on the matter. Funding bodies...

...member at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR...the NIR light coming from your source and to your camera.  Two-photon image of miRFP670nano3-expressing...reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads to less interference...al., 2018). For detection of protein-protein interactions, we recommend using NIR split reporters based...detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11, 641-644 (2014). Filonov, G....reporter for in vivo imaging of protein-protein interactions. Cell Chem Biol 20, 1078-1086 (2013). Shcherbakova...

...that only 11% of the academic research in the literature was reproducible by their groups. Finally results...copes with the growing amounts of digital data generated in labs. While many labs approach the situation...are available on the market today, ranging from general note taking tools, to more scientific field-based...From pictures to excel sheets, researchers today generate vast amounts of digital data during their work...within your lab? In every lab, there are certain hierarchies and restrictions to data access. If these are...walk you through your ELN’s features and this interaction will give you an idea of how responsive and supportive...

...candidate regulatory kinase is known, it’s possible to generate non-phosphorylated recombinant protein and perform...Creating phosphoserine-incorporated proteins The generation of pSer-incorporated proteins requires a molecular...position. Practical tips for your experiments To generate as much pSer-incorporated protein of interest ...be optimized for parameters such as induction temperature and length of induction, which will vary between...between different proteins. Beyond these standard considerations, it is important to determine how much phosphorserine... effects of pSer on enzyme function, protein interaction, etc. by comparison to non-phosphorylated or ...

...suggests there are hundreds. This only includes general lab strains designed for subcloning or protein ...ATPase that is essential for recombination and general DNA repair Reduces plasmid recombination, increases...Δleu mtl1 DH10B Streptomycin MC1061 derivative. General cloning and storage, blue/white screening, leucine...)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ- DH5alpha   General cloning and storage of common plasmids, blue/white...galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109   General cloning and plasmid maintenance, blue/white screening...(TetR) TOP10 Streptomycin MC1061 derivative. General cloning and storage, blue/white screening. F- mcrA...

...produce molecular biological tools like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins... elucidating the molecular mechanisms behind interactions between crops like potatoes and their microbial...that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass. Dr. Duedu would...construction of new plasmids. Dr. Duedu will also use polymerases produced using plasmids from Addgene to test ...

...unpredictable loud noises and flashing lights - that sent average, control mice into depression. In case you were... skeletal muscle expression of kynurenine aminotransferases, to enhance the conversion of kynurenine into...between the muscle and brain, which may have therapeutic implications, say Hyo Youl Moon and Henriette...psychiatric and cognitive conditions as well as neurodegenerative diseases.” I don’t know about you, but I think...Pettersson AT, Ferreira DM, Krook A, Barres R, Zierath JR, Erhardt S, Lindskog M, Ruas JL. doi: 10.1016...

...for general audiences. So far, we are beginning to make an impact on society and science literacy. We ... Addictive Brain and science communication for general audiences. How does The Addictive Brain address...science, especially disease related topics. Neurodegenerative and brain disorders are frequently overshadowed...“manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked...

...become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke University Medical Center...that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...researchers, and cloned them into lentiviral vectors to generate a kit which is now available at Addgene. “We called... cancer therapy...