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Addgene
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  1. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...expression at an opportune time or go all out with constitutive high level expression? Finally, you can add ...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... and form hydrogen bonds. This post focuses on "sticky end" TOPO (also called TOPO-TA) cloning; however...

  6. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under the same...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...The antibody to Myc was developed in 1985 and is still the most prevalent clone used today! Goat, chicken...

  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated recombination...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...NeuroMab Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes...

  20. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...shared in total? Just over 49,000 times! Their long-lasting popularity illustrates the utility these tools ...
Showing: 781 - 800 of 835 results