We narrowed to 800 results for: Des

1. 10 Basic tips for mammalian cell culture

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   Blog Post

   Published

   July 12, 2018, 1:09 p.m.

   ...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers...

2. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...investigators to identify specific clones with desirable properties, either initially, or using secondary...

3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...

4. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...components into mammalian cell lines is quite broad and includes several different methods, so we've summarized...

5. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   Blog Post

   Published

   Sept. 23, 2014, 3:05 p.m.

   ...the site. Like Labguru, Quartzy offers software designed with scientists workflow in mind and can offer...

6. Droplet Digital PCR for AAV Quantitation

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   Blog Post

   Published

   Jan. 16, 2020, 2:18 p.m.

   ... to a new plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix...

7. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   ...minigenome system Minigenome systems, such as the one described here, are extremely useful research tools. In ...

8. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...activation. Including a prey-only control also provides a baseline level of reporter activation. Vary ...

9. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   Published

   March 20, 2014, 3:04 p.m.

   ...that extra two miles which is way more fun. And despite those who may wish to tell you so – towel corners...

10. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    Published

    July 7, 2020, 1:15 p.m.

    ... plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and subsequent...

11. CRISPR 101: Any Base Transversion Editors

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    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...an abasic site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage...

12. How to Be an Excellent Trainee

    Type

    Blog Post

    Published

    Sept. 26, 2023, 1:15 p.m.

    ...will identify a ‘scientific mentor’: someone who provides career advice and offers direction on your scientific...

13. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...collaborators than you realize. Being at a university provides the perfect platform for practicing initiative...

14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ... this re-inversion issue. It became possible to design FLEx vectors (Flip-excision vectors) that only ...

15. Five Popular Model Organisms

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    Blog Post

    Published

    April 11, 2019, 12:39 p.m.

    ...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...

16. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

17. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...Biol 24, 758-766 e753 (2017). Baloban, M. et al. Designing brighter near-infrared fluorescent proteins: insights...

18. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...with a site mutation (R11G). This site mutation destabilizes the ligase, allowing for active biotin molecules...

19. Transferable Skills Guide: Time Management

    Type

    Blog Post

    Published

    Dec. 5, 2017, 1:52 p.m.

    ...if I had planned my day extensively, my lack of desire to go sit in the tissue culture hood may have prevented...

20. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    Published

    April 16, 2024, 1:15 p.m.

    ...Cell type-specific delivery by modular envelope design. Nature Communications, 14(1), Article 1. https...