We narrowed to 813 results for: TIM
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TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....of course, only hypothetical at this point. More time and energy must be devoted to studying Cas3 and ...
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Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m.... Editing by John Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....available through Addgene, with more being added all the time. Keep in mind that libraries are only as good as... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....appropriate immunizing reversal drive at the same time to mitigate the potential effects of an accidental... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse and -rabbit... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....ancestry group. With the existing methodology, they estimate that the African ancestry-associated cancer dependencies... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....titration experiments might be helpful to achieve optimal results. It is important to note that this system... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m.... that a construct is functional before investing time and resources into making a stable cell line. When... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....that will work well with a given set of resources, time, and experimental needs. Since its invention in ... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....lentiviral minus strand in the 3’ LTR. These optimizations produced 90% accurate integration of multiplexed... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....by nonspecific complement mechanisms in serum. Sometimes this is due to the cells that the viral vectors... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....episomal vector system generated by the group is an optimized mixture of three episomal plasmids (Table 1) encoding... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....and Bagpipe. How does a gene get its name? Often times, a gene gets its name based on what happens to an... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....experiments. They’ve also helped researchers design and optimize cloning methodologies using counterselection. ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....libraries Overview of Library To better define the optimal conditions for CRISPR knockout screens, the Bassik... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....peripheral nervous system (PNS) targeting AAV Get 1st time AAV user tips from an AAV rookie Learn about some... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog PostPublishedSept. 9, 2025, 1:15 p.m....AIBS). Scientists at AIBS have allocated tremendous time and resources to generate highly specific tools ... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....vectors typically involves shuttle vectors, also sometimes called transfer vectors. The gene of interest ...