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Addgene
Showing: 781 - 800 of 866 results

  2. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ... their materials. The new office in London is dedicated to helping them find their way to new scientific... then she has worked in the the UK, California, Africa, and Australia, viewing science as a global collaboration...

  3. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...CRISPR array. The CRISPR array is composed of identical repeat sequences and variable spacer sequences...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

  4. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...Their work involves coordination and can require technical mastery. Scientists need to be doers for a long...in the way of the doers and by ensuring good communication. A manager must both hire and fire to make sure...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR...puromycin selection. You could then confirm this purification by performing a genotyping assay (such as Sanger...

  6. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving a great...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for...

  7. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...contains the proteins needed for the virus to replicate,  and the transfer plasmid which contains the ... sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ITRs due to their secondary...

  9. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...

  10. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...gene encodes a sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they...models carrying different SOX10 mutations. More specifically, they’ve proposed to use plasmids with modified...

  11. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...positions posted on recruitment websites, this job specifically looked for a researcher with 3 years+ of cancer...on the marketing or business background of the applicant. However, although this position did look for ...

  12. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Christopher Harvey’s lab. According to their publication (Chettih & Harvey, 2019), “this localization ...Recombinase AAV Targeting and manipulating specific, genetically-defined neuronal populations with intersectional...

  13. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...FiercePharma), or sign up to be alerted about their new publications via Pubmed, so you can congratulate them when...really understood what I was going through. (Parenthetically, when I called the lab to ask my benchmate ...

  15. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...pipetting experience. However, after a few months of dedicated time at BosLab, he became our bacterial transformation... for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from UNC-Chapel Hill and...

  16. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ...

  18. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...end joining (NHEJ). In these reporters, editing typically results in a change in the fluorescent readout...stimulated homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074...

  19. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... the following, which are later printed for my physical lab notebook, along with any figures generated.... She is particularly interested in science communication and epigenetics. Follow her on twitter @kc_palozola...

  20. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...plasmid. You should also verify the strain and the efficacy of your competent cells (when they get old, they...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...
Showing: 781 - 800 of 866 results