We narrowed to 851 results for: nts
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TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....recombinases were also active in mammalian cells. In experiments, B3 and KD were shown to be functional in cultured...
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London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....information and I believe that a lot of European students and researchers are craving, like I was, for a... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m....protein-coding genes as well as non-coding genetic elements.” These lentiviral libraries and their demonstrated... -
New Viral Vectors - Fall 2024
TypeBlog PostPublishedOct. 3, 2024, 1:15 p.m.... prep packaged in the AAV9-X1.1 serotype. In rodents, AAV9-X1.1 enables expression in brain endothelial... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....labs have created Cas9 enzymes with looser PAM requirements, which are available from Addgene! RNA crRNA... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m....chromatography requires proper folding of the tag, which prevents insoluble fusion proteins from being purified ... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....classical complement pathway, starting a cascade of events that results in the pores being opened in the pathogen... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m.... line knock-ins of patient mutations which are variants of unknown significance – mutations with unclear... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....what types of jobs you are interested in in the comments. They could be featured in future career blog ... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m..... Don’t discount job postings looking for PhD applicants since many will also accept applications from... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....an essential amino acid), and a plasmid that complements the auxotrophy (i.e., synthesizes the critical... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....plasmid and take a look at each of its parts. Key elements of the AAV packaging plasmid The packaging plasmid... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will likely end up in a ... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....of my time meeting with and talking to graduate students, post-docs, and PIs. I won’t pretend that that... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....successful because you are not fulfilling the requirements of the current task. Most of us start as doers... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....find science writers who started out as graduate students themselves. Ask them if they’d be willing to meet... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....Biological Tools Viral Vectors 101: Viral Vector Elements More resources from Addgene The AAV Data Hub Addgene's... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....species. While this will not affect single-plexed experiments, it needs to be addressed when multiplexing. ... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m.... the beans on all things HEK, from the first experiments isolating the cell line all the way to modern... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....AAAS offers a summer program that gives graduate students an opportunity to work in radio, print journalism...