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...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

...the SPARC system, attP sites are located on either side of a stop cassette, upstream of an effector gene...Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Nature Methods...

...Intelligence or to hone delegation skills, for example. So aside from a focus on removing obstacles, what makes a... (this actually varies, a manager does best to identify the work style of each team member) Need challenging...

...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van ...

...CRISPOR, a tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 ...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi...

...Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers...

...sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...sequencing projects were launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year...Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links...

...primary antibodies can be expensive and may not provide enough signal to visualize low-abundance targets...your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your primary ...

...aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition...

...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different...

... Di Ventura on Twitter, you might have seen the video of her singing about the paper recently published... transcription from the PBAD promoter. “AraC is widely used in synthetic biology, biotechnology, and almost...

...make them decide to read (or listen or watch) further.  Second, the introduction needs to provide enough ...

...cells to take up protein directly? It’s not a new idea, but it comes with a few caveats. The most common...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...

...control AAV inventory to give scientists access to a wide array of promoters and fluorophores for validating...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

... schedule tools How to write a lot: A practical guide by Paula J. Silvia  This book has tips for how to...suggestions. De-jargonizer The De-jargonizer can identify jargon in your writing. While it’s not possible...

...Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental...Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...

...Professor Max Krummel from UCSF showed some impressive videos of these interactions (Ref 3). You can find useful...tips on in vivo imaging in our Fluorescent Protein Guide. Throughout the conference aspirin and the use of...

...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et... protein. This technique is very similar to the widely used FRET, but it eliminates the need for an excitation...