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...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different...

... Di Ventura on Twitter, you might have seen the video of her singing about the paper recently published... transcription from the PBAD promoter. “AraC is widely used in synthetic biology, biotechnology, and almost...

...sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...sequencing projects were launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year...Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links...

...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van ...

...make them decide to read (or listen or watch) further.  Second, the introduction needs to provide enough ...

...cells to take up protein directly? It’s not a new idea, but it comes with a few caveats. The most common...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...

... schedule tools How to write a lot: A practical guide by Paula J. Silvia  This book has tips for how to...suggestions. De-jargonizer The De-jargonizer can identify jargon in your writing. While it’s not possible...

...control AAV inventory to give scientists access to a wide array of promoters and fluorophores for validating...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental...Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...

...inhibits over 18,000 human genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...sequencing primers, and hand deliver them to our Sanger provider. These results come back within 8-12 hours and...and meet our QC standards. If any concerns are identified, we can quickly put a hold on all orders and ...

...Professor Max Krummel from UCSF showed some impressive videos of these interactions (Ref 3). You can find useful...tips on in vivo imaging in our Fluorescent Protein Guide. Throughout the conference aspirin and the use of...

...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et... protein. This technique is very similar to the widely used FRET, but it eliminates the need for an excitation...

...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...incorporating chemically inducible approaches to provide temporal control, this new system, Fkbp/Frb Inducible...

...synthesized endogenous proteins in situ. Now FUNCAT-PLA provides both quantitative and spatio-temporal information...protein synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of...

...messengers. Optical control of kinase activity can provide greater spatiotemporal resolution than pharmacological...vanishing, and pa= photoactivation) domains on either side of the active site of a constitutively active MEK1...

...the respective hairpin binding domain. Pairs of identical hairpin sequences specify the primary colors blue... colors and a white composite. gRNAs with two identical hairpin binding domains produce blue, green, or...

... Kramer L, Campbell RE. Structure-and mechanism-guided design of single fluorescent protein-based biosensors...1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral...