We narrowed to 845 results for: LEA

1. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   Published

   Aug. 10, 2021, 1:15 p.m.

   ...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...

2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   Published

   Feb. 7, 2019, 2:16 p.m.

   ...the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find...

3. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...

4. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   Published

   May 29, 2014, 1:29 p.m.

   ...: An Inside Look at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. Resources...

5. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...quantified, and validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies...

6. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https...

7. Getting to Know Expansion Microscopy

   Type

   Blog Post

   Published

   June 20, 2023, 1:15 p.m.

   ...This limitation may be the both most obvious and least flexible one. Tissue expansion is not compatible...

8. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA...

9. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ... their minds, but I think many of the students learned something. Based on the initial guidelines I received...

10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter...

11. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ...

12. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...the goal of the experiment Mammalian cells At least one of the following: to stably transfect the vector...

13. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...Created with BioRender.com. Inosine excision leads to the first adenine transversion editors Uracil...

14. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    Published

    June 20, 2024, 1:15 p.m.

    ... chromosomal DNA ensure that the helicases are released from the DNA once the replication forks reach ...

15. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

16. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    .... It’s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your ...

17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know...

18. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ... about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic biology in Natural...

19. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    Published

    March 28, 2024, 1:15 p.m.

    ...integrin α4β1 required and conferred less force, leading cells to spread into symmetrical circles. While...

20. Five Popular Model Organisms

    Type

    Blog Post

    Published

    April 11, 2019, 12:39 p.m.

    ...primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy...