We narrowed to 839 results for: LEA
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TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA...
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SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m.... their minds, but I think many of the students learned something. Based on the initial guidelines I received... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....the goal of the experiment Mammalian cells At least one of the following: to stably transfect the vector... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....Created with BioRender.com. Inosine excision leads to the first adenine transversion editors Uracil... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m.... chromosomal DNA ensure that the helicases are released from the DNA once the replication forks reach ... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m..... It’s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your ... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m.... about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic biology in Natural... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....integrin α4β1 required and conferred less force, leading cells to spread into symmetrical circles. While... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m....Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz, N., Beggs, A. H., Wagers, A. J... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....PMC4737176. Additional Resources on the Addgene Blog Learn New Plasmid Cloning Techniques Pushing the Limits... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless: genetic... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m.... by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....structural understanding of your protein that can lead to novel insights about its biology and its behavior... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....slightly more expensive gradient gel, which will allow clear separation of proteins of different sizes. Table...