We narrowed to 849 results for: NTS

1. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   Published

   Feb. 19, 2019, 1:36 p.m.

   ...create new programmatic content as well as help our students succeed whether that be academically or on the...

2. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   Published

   April 20, 2023, 4 p.m.

   ...charades, I doubt anyone but me remembers any of the incidents above–and the dumpster noises were just last week...

3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...can eliminate variability between the different components. When your pathway needs precise fine-tuning ...

4. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...the best.” Besides working through the tough assignments, the skills that Susan  finds important for her...

5. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   Published

   Jan. 11, 2017, 3:30 p.m.

   ...their position and decided to allow individual departments to go ahead with the raises as they see fit, ...

6. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...your results are viable for translation to human patients. Picking receptors The receptor you choose is ...

7. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   Published

   June 25, 2020, 1:15 p.m.

   ...Shannon Rinaldi values loving what she does. Her parents told her that if she found something she loves ...

8. Pooled Library Amplifications

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   Blog Post

   Published

   May 30, 2024, 1:30 p.m.

   ... Genome-wide Inhibition Library. DNA and cell amounts After deciding on a cell type, you will need to...

9. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...cytometry has the ability to generate incredible amounts of data from a single experiment. Although there...

10. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...

11. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...found this to be particularly challenging. As PhD students and postdocs we are used to doing (almost) everything...

12. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

13. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...Nanobodies also simplify multicolor staining experiments because they don’t require using primary antibodies...

14. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ... the challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne...

15. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

16. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    .... You can get your bacteria to produce massive amounts of protein, but if it’s not folded correctly, there...

17. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ... fluorescent protein to use in your upcoming experiments. In the meantime, if you haven't checked out ...

18. Hot Plasmids: Fall 2024

    Type

    Blog Post

    Published

    Nov. 21, 2024, 1:30 p.m.

    ...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...

19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows for the ...

20. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...due to both on- and off-target effects, but also presents a strategy to control for these effects. By testing...