We narrowed to 851 results for: nts

1. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...including hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So ...

2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ... order, or every combination of materials and treatments is all too often glossed over. This is why it...

3. Developing a cold tolerant E. coli using specialized chaperones

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   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...thrive, in the face of some of Earth’s harshest environments: corrosive pH ranges, crushing pressures, near-melting...

4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...can eliminate variability between the different components. When your pathway needs precise fine-tuning ...

5. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...the best.” Besides working through the tough assignments, the skills that Susan  finds important for her...

6. Transferable Skills Guide: Public Speaking

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   Blog Post

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   April 20, 2023, 4 p.m.

   ...charades, I doubt anyone but me remembers any of the incidents above–and the dumpster noises were just last week...

7. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   Blog Post

   Published

   Jan. 11, 2017, 3:30 p.m.

   ...their position and decided to allow individual departments to go ahead with the raises as they see fit, ...

8. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   Blog Post

   Published

   June 25, 2020, 1:15 p.m.

   ...Shannon Rinaldi values loving what she does. Her parents told her that if she found something she loves ...

9. Viral Vectors 101: Chemogenetics

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   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...your results are viable for translation to human patients. Picking receptors The receptor you choose is ...

10. Transferable Skills Guide: Managing a Team

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    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...found this to be particularly challenging. As PhD students and postdocs we are used to doing (almost) everything...

11. Transferable Skills: Negotiation

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    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...

12. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...cytometry has the ability to generate incredible amounts of data from a single experiment. Although there...

13. Pooled Library Amplifications

    Type

    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ... Genome-wide Inhibition Library. DNA and cell amounts After deciding on a cell type, you will need to...

14. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

15. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...Nanobodies also simplify multicolor staining experiments because they don’t require using primary antibodies...

16. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ... the challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne...

17. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

18. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    .... You can get your bacteria to produce massive amounts of protein, but if it’s not folded correctly, there...

19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ... fluorescent protein to use in your upcoming experiments. In the meantime, if you haven't checked out ...

20. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...due to both on- and off-target effects, but also presents a strategy to control for these effects. By testing...