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...Farewell 2019, and hello 2020! As we're gearing up to start a new decade, we’re also looking back at the last...

... collection of genes that can provide a useful starting point for developing a model and an assay to test...

... terms any programming noob (like myself) can understand. You’ll also find actionable advice on how to...

...series on Management for scientists. This second installment in the Management for Scientists series will ...

...learning and for expanding your web of connections. A mistake made by many novice networkers has a potential ...

...system. These plasmids can also help scientists understand how a given gene is involved in a complex pathway...

...labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in human pluripotent...

... Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References and resources...

...sequencing. We then copy the contents of the resulting FASTA file into the desktop version of Snapgene to visualize...

...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...

...data by biomedical researchers and ready access to stably archived large-scale datasets by computational ...

...vitro and have emerged as a powerful model for understanding human physiology, development, and disease. ...

... suited for their primary application. Ready to start tagging? We are here to help with vectors, protocols...

...built-in primer design tools, but here are a few stand-alone sites for general primer design, as well as...

...Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre...

...Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

... early years of sdAbs development, the process started with immunizing a camelid (usually llamas or alpacas...

...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be....

...proficiency, communicate your work, maintain ethical standards and interact professionally with other scientists...

... much-needed community resource. Instead of painstakingly searching for experimental details in research...