...secondary considerations to validation, application specificity, and experimental considerations - no other...rarely work for every assay. Antibodies that are generated against a linear antigen tend to work very well...permeabilization will influence how the antibody interacts with its target and consequently the quality of...isolate an antibody’s immune complexes and confirm interacting partners by mass spectrometry (Uhlen, 2016). ...

...means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or Fab...the protein will interact with other components of your experiment. These considerations will help you narrow...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ...

...repeating before you can finally dive deep into generating the data you’re excited about. When we were ...increase dramatically if you are piecing together several PCR products together. And this assumes you did...cost per stab has increased 50% since 2019, while overall inflation of USD has gone up 19.8% in the same ...making one plasmid at a time, try constructing several plasmids in parallel. That way, you can run one...help scientists save even more!) If you assume an average of 5 hours of hands-on time per plasmid, Addgene...

...genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries that knock out ...themselves incredibly useful for the efficient generation of individual (and multiple) genetic perturbations... al., 2015; Wong et al., 2016). CombiGEM uses iterative one-pot reactions with straightforward restriction...functionally characterize combinatorial gene knockouts generated via multiplexed gRNA expression (Wong et al., ...gRNA targeting sequences, you can then readily generate your barcoded gRNA library sequences via oligo...

...Collection at Coriell. More information and images generated from these cell lines can be found in the Cell...functional aspects of the protein gleaned from the literature and engagement with researchers who study the...find out more about gene edited hiPS cell lines generated using the Allen Institute for Cell Science Plasmid...Drosophila cells, and C. elegans. In addition to generating fluorescent protein fusions, plasmids are available...find out more about gene edited hiPS cell lines generated using these plasmids, please visit the Cell Catalog...

... preparation of the tissue sample, several washes, and temperature changes to visualize the expression...to study gene expression and functions of key regeneration-related genes in four inexpensive and commercially...linearized DNA templates which they can easily generate off of a circular plasmid via PCR. Typically, ...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

...interviews, however, were more structured. I met with several individual and/or small groups, usually starting...interviewer,” you can find lists that have lots of general questions about job responsibilities, performance... an opportunity to demonstrate technical and transferable skills that make you qualified for the position...position. Highlight the outcomes or deliverables of your work, such as improving a process or procedure, ... pursuing a particular line of research is a deliverable. Find 10 steps to a perfect science talk Be prepared...

...dimeric nature can interfere with some applications. Several labs have been working to produce monomeric versions... under CC-BY license.   The Miyawaki lab generated a series of StayGold mutants and identified a ...mentioning that Kiryl Piatkevich and colleagues generated yet another monomeric StayGold variant (Piatkevich...primates. The authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco... brains. Figure 3: Overview of library generation and screening that led to identification and ...

...recognize multiple isotypes from a species, like a general anti-mouse antibody. Others recognize specific ...better choice for a mouse monoclonal IgG2a than a general anti-mouse IgG. In a broadly specific secondary...different species.      Table 1: Isotype considerations when choosing antibodies   Complexity is...tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex with ...narrowly specific secondary antibodies rather than generally reactive ones. And make sure to check for any ...

...Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR) symposia held across the...and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments promise...the passing and upcoming implementation of the Federal Labor Standards Act (FLSA) which mandates that ...different departments across the university and alterations to the student-university relationship, along...2. Taylor, Justin Q., et al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed...