We narrowed to 858 results for: NTS

... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

...Nanobodies also simplify multicolor staining experiments because they don’t require using primary antibodies...

... the challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne...

...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

.... You can get your bacteria to produce massive amounts of protein, but if it’s not folded correctly, there...

... fluorescent protein to use in your upcoming experiments. In the meantime, if you haven't checked out ...

...due to both on- and off-target effects, but also presents a strategy to control for these effects. By testing...

...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...

...could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows for the ...

...appreciate the opportunity to share these invaluable reagents with the scientific community. Note: Cary Valley...

...King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...

...Although the DE3/T7 RNAP system works well for most experiments, the lac promoter can “leak,” meaning that a ...

...hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly to other...

...biosensors NIR reporters can tell us about cellular events via  fluorescence changes occurring due to protein...

...surface of the CD8+ T cells and gate on the positive events for your flow analysis. However, certain downstream...

...strains, especially mutations that make major improvements such as those that increase plasmid yield and...

... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors ...

...directed differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors...