We narrowed to 871 results for: sta

1. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...references and web links from my first post and start designing your first fly experiment! Many thanks...

2. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   Published

   April 19, 2017, 3:25 p.m.

   ...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....

3. New and Upcoming Viral Vectors - September 2019

   Type

   Blog Post

   Published

   Sept. 12, 2019, 1 p.m.

   ...neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal circuits....

4. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   Published

   Aug. 28, 2014, 3:34 p.m.

   ... bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI)...

5. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...//doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, ...

6. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   Published

   Aug. 19, 2025, 1:15 p.m.

   ... GCaMP series (e.g., jGCaMP8) remains the gold standard for green fluorescence, while red indicators jRGECO1a...

7. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite...

8. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this...

9. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...that this enzyme does not cleave at a predictable distance from the targeted region. Konermann et al. show...

10. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...what goes into ‘making virus’ or what gag and pol stand for, we demystify all components of viral vectors...

11. Viral Vectors 101: Biosensors

    Type

    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ...Biosensors tend to be more reproducible when used as stable systems, rather than transiently overexpressing...

12. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    Published

    Nov. 19, 2019, 2:08 p.m.

    ...OptoNB can’t bind, it localizes to the cytosol. To start, the team inserted AsLOV2 into conserved sites in...

13. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,...

14. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ....org) in the past few years.   Figure 1: Stand aside, CERN — it's a new age of accelerating science...

15. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...workshop was well received, and then when the team started brainstorming ideas for the next workshop, they...

16. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations, or other...

17. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...aa 155), includes I152L in N-terminal fragment Constant rate of p53 tetramerization in response to DNA...

18. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used...

19. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...organism where deletion strains are available. Then, staff or students clone similar sequences from a less-...

20. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away does. ...