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...popular screening library is the MORF library, which stands for Multiplexed Overexpression of Regulatory Factors...

... much-needed community resource. Instead of painstakingly searching for experimental details in research...

...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

...neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal circuits....

...references and web links from my first post and start designing your first fly experiment! Many thanks...

...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....

...genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first author of the cystic...

... bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI)...

...that this enzyme does not cleave at a predictable distance from the targeted region. Konermann et al. show...

...OptoNB can’t bind, it localizes to the cytosol. To start, the team inserted AsLOV2 into conserved sites in...

...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite...

...//doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, ...

...what goes into ‘making virus’ or what gag and pol stand for, we demystify all components of viral vectors...

...Biosensors tend to be more reproducible when used as stable systems, rather than transiently overexpressing...

... GCaMP series (e.g., jGCaMP8) remains the gold standard for green fluorescence, while red indicators jRGECO1a...

...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this...

...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,...

...workshop was well received, and then when the team started brainstorming ideas for the next workshop, they...

....org) in the past few years.   Figure 1: Stand aside, CERN — it's a new age of accelerating science...

...method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations, or other...