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...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be....

...proficiency, communicate your work, maintain ethical standards and interact professionally with other scientists...

...popular screening library is the MORF library, which stands for Multiplexed Overexpression of Regulatory Factors...

...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

... much-needed community resource. Instead of painstakingly searching for experimental details in research...

...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

...genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first author of the cystic...

...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....

...references and web links from my first post and start designing your first fly experiment! Many thanks...

...neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal circuits....

... bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI)...

... GCaMP series (e.g., jGCaMP8) remains the gold standard for green fluorescence, while red indicators jRGECO1a...

...what goes into ‘making virus’ or what gag and pol stand for, we demystify all components of viral vectors...

...Biosensors tend to be more reproducible when used as stable systems, rather than transiently overexpressing...

...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite...

...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this...

...that this enzyme does not cleave at a predictable distance from the targeted region. Konermann et al. show...

...//doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, ...

...OptoNB can’t bind, it localizes to the cytosol. To start, the team inserted AsLOV2 into conserved sites in...

...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,...