We narrowed to 874 results for: sta

1. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

   Type

   Blog Post

   Published

   July 7, 2015, 3:36 p.m.

   ...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

2. Using CRISPR/Cas9 to Edit Disease Out of the Genome

   Type

   Blog Post

   Published

   June 23, 2022, 1:15 p.m.

   ...genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first author of the cystic...

3. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   Published

   April 19, 2017, 3:25 p.m.

   ...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....

4. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...references and web links from my first post and start designing your first fly experiment! Many thanks...

5. New and Upcoming Viral Vectors - September 2019

   Type

   Blog Post

   Published

   Sept. 12, 2019, 1 p.m.

   ...neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal circuits....

6. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   Published

   Aug. 28, 2014, 3:34 p.m.

   ... bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI)...

7. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   Published

   Aug. 19, 2025, 1:15 p.m.

   ... GCaMP series (e.g., jGCaMP8) remains the gold standard for green fluorescence, while red indicators jRGECO1a...

8. Viral Vectors 101: Viral Vector Elements

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   Blog Post

   Published

   July 25, 2023, 1 p.m.

   ...what goes into ‘making virus’ or what gag and pol stand for, we demystify all components of viral vectors...

9. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...Biosensors tend to be more reproducible when used as stable systems, rather than transiently overexpressing...

10. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...//doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, ...

11. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...that this enzyme does not cleave at a predictable distance from the targeted region. Konermann et al. show...

12. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite...

13. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this...

14. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,...

15. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    Published

    Nov. 19, 2019, 2:08 p.m.

    ...OptoNB can’t bind, it localizes to the cytosol. To start, the team inserted AsLOV2 into conserved sites in...

16. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ....org) in the past few years.   Figure 1: Stand aside, CERN — it's a new age of accelerating science...

17. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...workshop was well received, and then when the team started brainstorming ideas for the next workshop, they...

18. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations, or other...

19. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away does. ...

20. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...