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We narrowed to 837 results for: Eras

Showing: 431 - 440 of 837 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries that knock out ...themselves incredibly useful for the efficient generation of individual (and multiple) genetic perturbations... al., 2015; Wong et al., 2016). CombiGEM uses iterative one-pot reactions with straightforward restriction...functionally characterize combinatorial gene knockouts generated via multiplexed gRNA expression (Wong et al., ...gRNA targeting sequences, you can then readily generate your barcoded gRNA library sequences via oligo...

  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...interviews, however, were more structured. I met with several individual and/or small groups, usually starting...interviewer,” you can find lists that have lots of general questions about job responsibilities, performance... an opportunity to demonstrate technical and transferable skills that make you qualified for the position...position. Highlight the outcomes or deliverables of your work, such as improving a process or procedure, ... pursuing a particular line of research is a deliverable. Find 10 steps to a perfect science talk Be prepared...

  3. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ... preparation of the tissue sample, several washes, and temperature changes to visualize the expression...to study gene expression and functions of key regeneration-related genes in four inexpensive and commercially...linearized DNA templates which they can easily generate off of a circular plasmid via PCR. Typically, ...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

  4. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...Collection at Coriell. More information and images generated from these cell lines can be found in the Cell...functional aspects of the protein gleaned from the literature and engagement with researchers who study the...find out more about gene edited hiPS cell lines generated using the Allen Institute for Cell Science Plasmid...Drosophila cells, and C. elegans. In addition to generating fluorescent protein fusions, plasmids are available...find out more about gene edited hiPS cell lines generated using these plasmids, please visit the Cell Catalog...

  5. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...dimeric nature can interfere with some applications. Several labs have been working to produce monomeric versions... under CC-BY license.   The Miyawaki lab generated a series of StayGold mutants and identified a ...mentioning that Kiryl Piatkevich and colleagues generated yet another monomeric StayGold variant (Piatkevich...primates. The authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco... brains. Figure 3: Overview of library generation and screening that led to identification and ...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...recognize multiple isotypes from a species, like a general anti-mouse antibody. Others recognize specific ...better choice for a mouse monoclonal IgG2a than a general anti-mouse IgG. In a broadly specific secondary...different species.      Table 1: Isotype considerations when choosing antibodies   Complexity is...tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex with ...narrowly specific secondary antibodies rather than generally reactive ones. And make sure to check for any ...

  7. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR) symposia held across the...and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments promise...the passing and upcoming implementation of the Federal Labor Standards Act (FLSA) which mandates that ...different departments across the university and alterations to the student-university relationship, along...2. Taylor, Justin Q., et al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed...

  8. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...facilitate effective teamwork, manage day to day operations within their workplace, and pursue leadership...need regular re-direction and alignment with the overall mission of the NPA. You must accommodate many different...learnt to try and bring a group of people to an overall consensus and developed negotiation skills that...would be useful in many careers. I learnt that the overall voice may not always be perfectly aligned with ...to think about their career options, gaining transferable skills such as leadership skills can only assist...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...how best to prepare. Maybe you’re a presentation veteran, but have difficulty answering questions. Or perhaps... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...topics,” so be sure to be well-read on the recent literature in your field. Take your time: We often get so...making it difficult to see the actual poster or interact with the presenter. If you don’t have time to ...at the University of Arizona. She studies the interaction between Human Papillomavirus and the innate immune...

  10. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...the spotlight — Cas3. Cas3 can induce genomic alterations on a scale that other Cas effectors can’t, while...maintaining relatively high efficiency. These alterations are achieved through DNA degradation, thanks ...that the cell can use to repair. Figure 3: General overview of the Cascade-Cas3 mechanism. Created... gRNA. Cas3 appears to have fewer off-target alterations, likely due to the multi-Cas mechanism. These...has the potential to be incredibly useful in therapeutics. Imagine deleting a problematic copy of a gene...
Showing: 431 - 440 of 837 results