...facilitate effective teamwork, manage day to day operations within their workplace, and pursue leadership...need regular re-direction and alignment with the overall mission of the NPA. You must accommodate many different...learnt to try and bring a group of people to an overall consensus and developed negotiation skills that...would be useful in many careers. I learnt that the overall voice may not always be perfectly aligned with ...to think about their career options, gaining transferable skills such as leadership skills can only assist...

...how best to prepare. Maybe you’re a presentation veteran, but have difficulty answering questions. Or perhaps... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...topics,” so be sure to be well-read on the recent literature in your field. Take your time: We often get so...making it difficult to see the actual poster or interact with the presenter. If you don’t have time to ...at the University of Arizona. She studies the interaction between Human Papillomavirus and the innate immune...

...be able to tell you the laser configuration. In general, if two fluorescent markers are read by the same...to become a perpendicular vertical line.     In general, most spillover between samples can be fixed with...anyone just starting to use flow cytometry, it’s generally recommended to start compensating with single-... compensation panels below each plot.  The general rules of compensation apply to your experimental...research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician in ...

...the spotlight — Cas3. Cas3 can induce genomic alterations on a scale that other Cas effectors can’t, while...maintaining relatively high efficiency. These alterations are achieved through DNA degradation, thanks ...that the cell can use to repair. Figure 3: General overview of the Cascade-Cas3 mechanism. Created... gRNA. Cas3 appears to have fewer off-target alterations, likely due to the multi-Cas mechanism. These...has the potential to be incredibly useful in therapeutics. Imagine deleting a problematic copy of a gene...

...controversy, and the interactions of organic molecules like proteins with magnetic fields generally appears to ...magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random mutagenesis and screening...behavior has already been successfully reproduced by several other labs. Andrew and Maria expect that further-improved...tools.  Embracing science in the open The York Lab operates with a strong commitment to exploration and collaboration...

...light generated by sunlight to observe single gold particles with diameters less than 4nm. Lateral view...field-of-views (6). For more comprehensive reviews on several other LSFM configurations’ various features see...microscopes are less efficient than the modern cameras used in LSFM. In LSFM the laser light sheet, typically...microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged device...

...paCas9-1 using nMagC714 instead of nMagHigh1-C714, generating paCas9-2. This change did not significantly alter... alter the system’s efficiency at generating mutations when activated with light and lowered background...out the Cas9 domains in their split fusions and generate a photoactivatable nickase and a photoactivatable...protein due to being fused with high-affinity interacting proteins Coh2 and DocS, respectively. The use... the versatility of this approach. This study generated the first optically active saCas9 protein. Stop...Charpentier E, Möglich A (2013) Engineering of temperature- and light-switchable Cas9 variants. Nucleic ...

...Brunello library consists of 77,441 sgRNAs with an average of 4 sgRNAs per gene and 1,000 non-coding control...identifying strong hits in genetic screens, they do have several limitations. CRISPRko results in a complete loss... and the ORF overexpression library identified several overlapping genes, most genes were unique to the...Applications for the Root and Doench CRISPR libraries Overall the Root and Doench lab’s have proven their optimized..., Kendall R., et al. "Up, down, and out: next generation libraries for genome-wide CRISPRa, CRISPRi, and...

...formation on agar plates, less stable at high temperatures and in acidic environments.   Carbenicillin   ...mechanism. How to choose between the two? Carb is generally a ‘better’ antibiotic than amp: it is more stable...inactivates it. The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly...induce cell death by intercalating into DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance...., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial agents...

...hundreds of samples, with an average of 10 plasmids per deposit. Each week an average of 16 deposits is received...Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations, she also...stored right away. Every week, the lab performs an average of 140 transformations. The lab typically transforms...