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We narrowed to 837 results for: Eras

Showing: 441 - 450 of 837 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...controversy, and the interactions of organic molecules like proteins with magnetic fields generally appears to ...magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random mutagenesis and screening...behavior has already been successfully reproduced by several other labs. Andrew and Maria expect that further-improved...tools.  Embracing science in the open The York Lab operates with a strong commitment to exploration and collaboration...

  2. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...be able to tell you the laser configuration. In general, if two fluorescent markers are read by the same...to become a perpendicular vertical line.     In general, most spillover between samples can be fixed with...anyone just starting to use flow cytometry, it’s generally recommended to start compensating with single-... compensation panels below each plot.  The general rules of compensation apply to your experimental...research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician in ...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...light generated by sunlight to observe single gold particles with diameters less than 4nm. Lateral view...field-of-views (6). For more comprehensive reviews on several other LSFM configurations’ various features see...microscopes are less efficient than the modern cameras used in LSFM. In LSFM the laser light sheet, typically...microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged device...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...paCas9-1 using nMagC714 instead of nMagHigh1-C714, generating paCas9-2. This change did not significantly alter... alter the system’s efficiency at generating mutations when activated with light and lowered background...out the Cas9 domains in their split fusions and generate a photoactivatable nickase and a photoactivatable...protein due to being fused with high-affinity interacting proteins Coh2 and DocS, respectively. The use... the versatility of this approach. This study generated the first optically active saCas9 protein. Stop...Charpentier E, Möglich A (2013) Engineering of temperature- and light-switchable Cas9 variants. Nucleic ...

  5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...Brunello library consists of 77,441 sgRNAs with an average of 4 sgRNAs per gene and 1,000 non-coding control...identifying strong hits in genetic screens, they do have several limitations. CRISPRko results in a complete loss... and the ORF overexpression library identified several overlapping genes, most genes were unique to the...Applications for the Root and Doench CRISPR libraries Overall the Root and Doench lab’s have proven their optimized..., Kendall R., et al. "Up, down, and out: next generation libraries for genome-wide CRISPRa, CRISPRi, and...

  6. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...formation on agar plates, less stable at high temperatures and in acidic environments.   Carbenicillin   ...mechanism. How to choose between the two? Carb is generally a ‘better’ antibiotic than amp: it is more stable...inactivates it. The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly...induce cell death by intercalating into DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance...., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial agents...

  7. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...hundreds of samples, with an average of 10 plasmids per deposit. Each week an average of 16 deposits is received...Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations, she also...stored right away. Every week, the lab performs an average of 140 transformations. The lab typically transforms...

  8. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities, ... was a lot of blood, sweat, and, I’m guessing, literal tears (though none of the founders have EVER confirmed...to do, feel free to crowdsource! 2. Free food generally gets people to show up If you feel like you may... event planner)! If you have a very intricate itinerary, there is a higher likelihood that something will...

  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    .... Cas13a (previously referred to as C2c2), has several unique properties that further expand the CRISPR...other hand, uses only a short ~24 base crRNA that interacts with the Cas13a molecule through a uracil-rich...changes in the Cas13a molecule. Like Cas9, Cas13a tolerates single mismatches between the crRNA and target...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...knockdown to single gRNA expression. Although the collateral RNA cleavage response is well described in bacterial...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... An overview of peptide synthesis. A ribosome interacts with mRNA and charged tRNAs to build a peptide...codon usage among different organisms. There are several codons that encode the same amino acid. Each organism... be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall, codon optimization may not always be the optimal...in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is...critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https...
Showing: 441 - 450 of 837 results