...Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities, ... was a lot of blood, sweat, and, I’m guessing, literal tears (though none of the founders have EVER confirmed...to do, feel free to crowdsource! 2. Free food generally gets people to show up If you feel like you may... event planner)! If you have a very intricate itinerary, there is a higher likelihood that something will...

...can use your cover letter to explain why your transferable skills make you a good candidate for the position...experience or skills, often by pointing to your transferable skills. In the above example, if you don’t have...During my thesis work on [phenomenon X], I read several of your publications on [specific topic]. I am ...and grammatical errors. It does not need to be a literary masterpiece. For most jobs, the writing quality...professional. Always review grammar and spelling several times and ask someone else read your cover letter...

...their structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts...ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas campestris pv. campestris, X. campestris...Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae, Xanthomonas campestris   In addition to incorporating... the broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https://doi.org...

.... Cas13a (previously referred to as C2c2), has several unique properties that further expand the CRISPR...other hand, uses only a short ~24 base crRNA that interacts with the Cas13a molecule through a uracil-rich...changes in the Cas13a molecule. Like Cas9, Cas13a tolerates single mismatches between the crRNA and target...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...knockdown to single gRNA expression. Although the collateral RNA cleavage response is well described in bacterial...

... An overview of peptide synthesis. A ribosome interacts with mRNA and charged tRNAs to build a peptide...codon usage among different organisms. There are several codons that encode the same amino acid. Each organism... be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall, codon optimization may not always be the optimal...in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is...critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https...

...In their BioRxiv post, the authors generated and described several improved genetically encoded fluorescent...! Schematic of the evolution platform to generate TEV protease variants. Catalysis by TEV protease...markers can lead to non-specific labeling. They generated plasmids tagged with EB3 to mark the growing tips...plasmids containing different nanobodies (NB) were generated in pMAK:  pMAK461(NBαRabitb-IgG-Fc, Addgene #140699...

...they study (unless if you count normal human interaction for all of us studying human genes in test tubes...invited to attend. My job is to ensure that, in general, these labs all include materials sharing in their...Monarch butterfly food. They have chemical pathways generating interesting drug-like compounds especially cardenolides... heart disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous...Celina Juliano) This is a known stem cell and regeneration model. Little bits of hydra can grow back into...

...plasmid-based experiments. What are control plasmids? Generally, control plasmids help to ensure that the observed...constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with the test ...non-targeting control also assesses any effects of the general introduction of shRNA into your target cells. Positive...replicate controls: technical and biological. In general, technical replicates can be thought of as "plate...of Plasmid A in different aliquots of cells on several different days. Biological replicates control for...

...organized pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case...about our bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow...You can read it, and other case studies, on the Seqera website. ...

...communicator overall. Don’t Write in a Vacuum Your research papers should go through many iterations read ...Saturday Hack Day projects sought to make an interactive game that highlights just this point. As part... this, trainees need to be able to network and interact more with people who have taken a variety of career...write for a university publication, improving your general writing skills will help you in your academic publishing...