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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 837 results for: Eras

Showing: 451 - 460 of 837 results

  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...they study (unless if you count normal human interaction for all of us studying human genes in test tubes...invited to attend. My job is to ensure that, in general, these labs all include materials sharing in their...Monarch butterfly food. They have chemical pathways generating interesting drug-like compounds especially cardenolides... heart disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous...Celina Juliano) This is a known stem cell and regeneration model. Little bits of hydra can grow back into...

  2. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...can use your cover letter to explain why your transferable skills make you a good candidate for the position...experience or skills, often by pointing to your transferable skills. In the above example, if you don’t have...During my thesis work on [phenomenon X], I read several of your publications on [specific topic]. I am ...and grammatical errors. It does not need to be a literary masterpiece. For most jobs, the writing quality...professional. Always review grammar and spelling several times and ask someone else read your cover letter...

  3. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...their structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts...ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas campestris pv. campestris, X. campestris...Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae, Xanthomonas campestris   In addition to incorporating... the broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https://doi.org...

  4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    .... Cas13a (previously referred to as C2c2), has several unique properties that further expand the CRISPR...other hand, uses only a short ~24 base crRNA that interacts with the Cas13a molecule through a uracil-rich...changes in the Cas13a molecule. Like Cas9, Cas13a tolerates single mismatches between the crRNA and target...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...knockdown to single gRNA expression. Although the collateral RNA cleavage response is well described in bacterial...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...plasmid-based experiments. What are control plasmids? Generally, control plasmids help to ensure that the observed...constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with the test ...non-targeting control also assesses any effects of the general introduction of shRNA into your target cells. Positive...replicate controls: technical and biological. In general, technical replicates can be thought of as "plate...of Plasmid A in different aliquots of cells on several different days. Biological replicates control for...

  6. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...organized pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case...about our bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow...You can read it, and other case studies, on the Seqera website. ...

  7. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...communicator overall. Don’t Write in a Vacuum Your research papers should go through many iterations read ...Saturday Hack Day projects sought to make an interactive game that highlights just this point. As part... this, trainees need to be able to network and interact more with people who have taken a variety of career...write for a university publication, improving your general writing skills will help you in your academic publishing...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...coupling of Cre-lox with FLP-FRT recombination to generate additional recombination events, and the synthesis...and sea anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and...analysis. To achieve even cell labeling, they generated farnesylated derivatives of the fluorescent proteins...previously visible with Brainbow-1 and -2. The general structure of Brainbow-1.0 is retained in Brainbow...constructs are stably maintained through at least six generations. In addition to the neuronal pThy1-Brainbow constructs...

  9. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... would thrive on this level of attention and interaction (and I did). Some of the information you will...advisor. They are always traveling, or too busy to interact regularly with the newer lab members. You must...careers?    Will you be encouraged to develop transferable skills by attending career events or being involved...your own choosing? Does the PI keep up on the literature and pass on relevant papers? Will you have access...resources on the Addgene blog Check out our transferable skills guide Learn about careers in science ...

  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...twice the length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to increasing...which has been used in plasmid vaccine and gene therapy development to examine plasmid topologies (e.g....stationary phase. Growing the culture at a lower temperature such as 30°C or optimizing the incubation time...Fishel RA, James AA, Kolodner R. recA-independent general genetic recombination of plasmids. Nature. 1981...294184a0. PMID: 7029308. Goebel W, Helinski DR. Generation of higher multiple circular DNA forms in bacteria...
Showing: 451 - 460 of 837 results