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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 837 results for: Eras

Showing: 461 - 470 of 837 results

  1. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...started by designing a novel topology, then iteratively generated 3D models and amino acid sequences that ...topology of protein” diagrams, which show the interactions between amino acids in an approximate, two-dimensional...crosslinked peptides proposed as a scaffold for a new generation of drugs, all developed with their protein design...

  2. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ...ways of generating antibodies, for example through the use of less toxic adjuvants; 2) generating complex... The minimal fees from distribution funds our operation. Antibodies are glycoproteins that are part of...increased avidity to high profile antigens; and 4) generating mAbs against new cancer biomarkers. We believe...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...) (Miller et al., 2005). Although this tight interaction is not covalent, later work showed that introducing...ER and mitochondria reveals dynamic organelle interactions. a) Labeling scheme. b) COS-7 cell ER labeled...without any cloning at all! As mentioned above, several variants of these tags have been developed over...https://doi.org/10.1021/cb900062k Gautier, A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M.... Pick, H., Vogel, H., & Johnsson, K. (2003). A general method for the covalent labeling of fusion proteins...

  4. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ...not right for the development of phage-based therapeutics. After a brief stint at the venture capital ...Novophage's focus. The company went from developing therapeutics to developing phage-based tools for detecting...develop tests for food products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens...for Dr. Koeris to transition from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : ...

  5. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...are required to ensure that you get full library coverage in pooled library screens. Availability and Use...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR...of the many plasmids being distributed to and accelerating research in labs all around the world. We thank...Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR...

  6. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...of different conformations and thus potentially interact with and alter the proteins they label. In fact...chaperone Ydj1, both of which display sophisticated interactions with multiple other proteins. Again, little ...indicating that they compromised protein-protein interactions. If you’re looking to a tag a protein, inntags...tags of choice for examining protein-protein interactions, particularly in single-molecule or high-throughput...

  7. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ... the key component of most CRISPR systems and generates double strange breaks at target sites 5' of the...your 20 nucleotide guide sequence of choice (for generation of a gRNA that will target SpCas9 to the approrpriate... expression post transfection by western blot. Overall, this is a versatile mammalian expression vector...Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR...

  8. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...DayCon. DayCon is a one-day conference aimed at the general public that provides an accessible introduction...conference-style experience that was accessible to the general public both in terms of cost and content level....of disease, cancer, and viral infections. An interactive lunch with student presenters and board members... of shorter, more narrowly focused lectures, interactive panels, and a student poster session. All DayCon...

  9. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...available at Addgene, also gives the option to stack several expression cassettes onto a single T-DNA in subsequent...rounds, dramatically reducing the time required to generate multi-construct transgenic plants. In the most...choice, BsaI, which can be very sensitive to temperature fluctuations. He says their lab learned the hard...into the public domain in the process. “We had several motivations for making GreenGate available through...

  10. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...towards the objective is collected and focused onto a camera for visualization. Illuminating a sample in the...is excited at one time and captured in a single camera exposure. This is much faster than relying on raster...fast, sensitive detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus... excitation occurs through the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes... to a higher photon flux via the more efficient camera detector and lack of a light-reducing pinhole. ...
Showing: 461 - 470 of 837 results