We narrowed to 842 results for: Eras

... the key component of most CRISPR systems and generates double strange breaks at target sites 5' of the...your 20 nucleotide guide sequence of choice (for generation of a gRNA that will target SpCas9 to the approrpriate... expression post transfection by western blot. Overall, this is a versatile mammalian expression vector...Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR...

...DayCon. DayCon is a one-day conference aimed at the general public that provides an accessible introduction...conference-style experience that was accessible to the general public both in terms of cost and content level....of disease, cancer, and viral infections. An interactive lunch with student presenters and board members... of shorter, more narrowly focused lectures, interactive panels, and a student poster session. All DayCon...

...available at Addgene, also gives the option to stack several expression cassettes onto a single T-DNA in subsequent...rounds, dramatically reducing the time required to generate multi-construct transgenic plants. In the most...choice, BsaI, which can be very sensitive to temperature fluctuations. He says their lab learned the hard...into the public domain in the process. “We had several motivations for making GreenGate available through...

...towards the objective is collected and focused onto a camera for visualization. Illuminating a sample in the...is excited at one time and captured in a single camera exposure. This is much faster than relying on raster...fast, sensitive detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus... excitation occurs through the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes... to a higher photon flux via the more efficient camera detector and lack of a light-reducing pinhole. ...

...) (Miller et al., 2005). Although this tight interaction is not covalent, later work showed that introducing...ER and mitochondria reveals dynamic organelle interactions. a) Labeling scheme. b) COS-7 cell ER labeled...without any cloning at all! As mentioned above, several variants of these tags have been developed over...https://doi.org/10.1021/cb900062k  Gautier, A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M.... Pick, H., Vogel, H., & Johnsson, K. (2003). A general method for the covalent labeling of fusion proteins...

...s how science progresses after all, with each generation of scientists standing on the shoulders of their...Technology Policy issued a memorandum calling on Federal agencies with over $100 million in annual R&D expenditures...support increased public access to the results of federally-funded research. 2)      In June, the White House...Bohannon published an article describing a sting operation in which he submitted a phony paper to a list ...

...then formulate a plan to further develop your transferable skills and reach your career goals.      Although...subject matter discussed at the meeting, but the overall tone of the meeting is almost more important. As...Wunderlich Z, Estrada J, Park J, Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58... What Makes a Good Manager Anyway Developing Transferable Skills During Science Training ...

... the interactive photo graffiti wall. I had a lot of fun playing the prototype of an interactive game ...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists...

...opportunities, protocols, publications, and more to accelerate the pace of research into COVID-19. While this...opportunities, and community response initiatives. Accelerate the translational impact of research Universities...important for creating research synergies and accelerating the translational impact of research activities...Alliance at the CCRP2. Other groups have focused on iterating on methods for coronavirus research, sharing resources...

...from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding...about new recombinases suitable for this system. Gerald Rubin’s lab sought to make complex genome modifications...specific recombination site for each enzyme, were generated to test recombination efficiency. When expressed...PubMed. Reduction of Cre recombinase toxicity in proliferating Drosophila cells by estrogen-dependent activity...