...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...Purification of His fusion proteins from E. coli is generally more straightforward and of a higher purity than...washing. Nonetheless, this tag can be purified with several different resin types and is a fairly small tag...and other qualities of each tag class, they are generally best suited for their primary application. Ready...

...cell line collections websites and catalogues Literature searches utilizing Google Scholar alerts and ...feature to know if your lines may be especially vulnerable can be very informative. Spoiler alert: HeLa ...information on the known features and relevant literature, or to decide which line is best for your experiments...Josef Reischig.  Contributing to Cellosaurus Generated a new cell? Or finding errors in the available...

...behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA...body. Administering the drug once can result in several hours of neuronal excitation or inhibition. This...sustained activation period is beneficial for use in therapeutic or behavioral applications where drawn out effects... by Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology...

...of time, especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider...molecular biology like restriction enzymes and polymerases. In the situation where you need to use plasmid...Plasmid DNA can be stable at 4°C or even room temperature for a short period, and there are indications...

...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...lights (Baier et al., 2009).  Zebrafish behaviors Several behavioral assays can be done on zebrafish at 5...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit...circuit involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...

...receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However, the R206H mutation...including Activin A. A commercially developed therapeutic antibody that binds Activin A and interferes ...interferes with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic...experience is in both vaccine subunit design and therapeutic biologic design (ADCs) along with pre-clinical...

...the the University of Cambridge and within the general public. The society organises talks by prominent...graduate students, postdocs, faculty members, and the general public. Chemical Society in University College ...studying to earn a PhD. The society encourages interactions between undergraduates and PhD students so that...develop a range of talks for both students and the general public, career events, and networking meetups. ...

...included in organizational decision-making. It is imperative that employees are thanked for their work by ...but it is worth it. This requires a focus on deliverables. It does not mean a manager must allow his team...enough of a jerk to make the rest of the team miserable.   A good manager keeps these factors in mind...decisions, but that should not be the common mode of operating.  Managing scientists I should add a few special...

... concept that these clustered repeat sequences operated as a safeguard against bacteriophages did not ... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted...proteins then express and process the CRISPR loci to generate RNA, which guide a Cas nuclease to the specified...learned to circumvent the CRISPR/Cas defense by generating a single point mutation in the PAM sequence, ...

...publications—the pressure to produce is the same You get to interact with scientists just like you What is different...structures are common Small integrated teams, little hierarchy Real diversity and acceptance can be an issue...requirements for hiring more common Likelier to hire on general talent and not specific skills <- Can move between...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...