We narrowed to 729 results for: NSI

1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...naturally in camels, consist of a single heavy chain, and they are small and stable inside cells. Nanobodies...

2. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ...abundance before and after a treatment. The library consists of a pool of lentiviral vectors with roughly 73...populations, they found that similar barcodes consistently made up the majority of the resistant populations...

3. Corralling and Cornering the Goldilocks Antibody

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   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...scientists to confidently choose reliable and consistent antibodies for specific markers. The Screening...production methods    NeuroMab and others have an extensive library of monoclonal antibodies; Addgene collaborates...

4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ... toolkit from Benjamin Field’s lab. Special Considerations There are a few special exceptions to the usual...constructs or certain cell environments). Also consider that a protein fusion may disrupt functionality...

5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...   Let me leave you with a few practical considerations: Design your gRNA to overlap the CpG of interest...interest. Having the CpG directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked...

6. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...Polyclonal antibodies are produced in animals and consist of a mixture of antibodies recognizing many different...hybridomas.  First, the long-term stability, consistency between batches, and molecular definition of ...

7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ... example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter...any template DNA and primers.  After performing extensive QC on that sample, we’ll then load it on to our...

8. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...and mice with Nanoblades CRISPR has also been extensively used to create transgenic mice by microinjecting...post Resources on Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources...

9. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   Blog Post

   Published

   Aug. 6, 2019, 12:55 p.m.

   ...Special breeding cases Whether your mouse colony consists of genetically engineered mice with a single mutation...strains that have been crossed, there are many considerations for effectively breeding your mice. Because...

10. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ... crossing the blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies...response you aim to manipulate. It is important to consider that the same receptor can elicit different responses...