We narrowed to 526 results for: SHE

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...

...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

...antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies to resensitize...

...with a molecule of interest (the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group...

...about Cpf1 and check out our CRISPR guide for a refresher on CRISPR/Cas9.  Figure 1: Cpf1 is a single...

...structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore ...

...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...