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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 837 results for: Eras

Showing: 481 - 490 of 837 results

  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...Purification of His fusion proteins from E. coli is generally more straightforward and of a higher purity than...washing. Nonetheless, this tag can be purified with several different resin types and is a fairly small tag...and other qualities of each tag class, they are generally best suited for their primary application. Ready...

  2. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...cell line collections websites and catalogues Literature searches utilizing Google Scholar alerts and ...feature to know if your lines may be especially vulnerable can be very informative. Spoiler alert: HeLa ...information on the known features and relevant literature, or to decide which line is best for your experiments...Josef Reischig.  Contributing to Cellosaurus Generated a new cell? Or finding errors in the available...

  3. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However, the R206H mutation...including Activin A. A commercially developed therapeutic antibody that binds Activin A and interferes ...interferes with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic...experience is in both vaccine subunit design and therapeutic biologic design (ADCs) along with pre-clinical...

  4. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...the the University of Cambridge and within the general public. The society organises talks by prominent...graduate students, postdocs, faculty members, and the general public. Chemical Society in University College ...studying to earn a PhD. The society encourages interactions between undergraduates and PhD students so that...develop a range of talks for both students and the general public, career events, and networking meetups. ...

  5. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...included in organizational decision-making. It is imperative that employees are thanked for their work by ...but it is worth it. This requires a focus on deliverables. It does not mean a manager must allow his team...enough of a jerk to make the rest of the team miserable.   A good manager keeps these factors in mind...decisions, but that should not be the common mode of operating.  Managing scientists I should add a few special...

  6. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ... concept that these clustered repeat sequences operated as a safeguard against bacteriophages did not ... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted...proteins then express and process the CRISPR loci to generate RNA, which guide a Cas nuclease to the specified...learned to circumvent the CRISPR/Cas defense by generating a single point mutation in the PAM sequence, ...

  7. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...publications—the pressure to produce is the same You get to interact with scientists just like you What is different...structures are common Small integrated teams, little hierarchy Real diversity and acceptance can be an issue...requirements for hiring more common Likelier to hire on general talent and not specific skills <- Can move between...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...

  8. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...study which can be accessed here at bioRxiv. AAV2 interacts with the packaging cell's cell membrane When packaging...blocked following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose...    Despite observing a positive effect on our overall yield, we were unable to demonstrate that these...-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475. PubMed Central...

  9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...developed in David Liu’s lab in 2019, has added considerably more precision and flexibility to the CRISPR... repair favoring the edited strand. The first iteration of prime editor simply fused the wild-type Moloney...pairs for prime editing. Since PAM sites occur on average every ~8 base pairs on either DNA strand, many ...by double strand breaks has been widely used to generate precise changes. However, the efficiency of Cas9...system PE2, which had prime editing efficiencies on average 2.3- to 5.1-fold (though up to 45-fold) higher ...

  10. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...cell survival or proliferation, an additional challenge is that only partial or ephemeral knockdown will...from the manufacturer and/or in the published literature. Note that the antibody being published doesn... used in combination with other approaches. If several cell lines express the target at different levels...contains information about the labeling pattern of several cell lines using these two antibodies. This is ...
Showing: 481 - 490 of 837 results