...publications—the pressure to produce is the same You get to interact with scientists just like you What is different...structures are common Small integrated teams, little hierarchy Real diversity and acceptance can be an issue...requirements for hiring more common Likelier to hire on general talent and not specific skills <- Can move between...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...

...developed in David Liu’s lab in 2019, has added considerably more precision and flexibility to the CRISPR... repair favoring the edited strand. The first iteration of prime editor simply fused the wild-type Moloney...pairs for prime editing. Since PAM sites occur on average every ~8 base pairs on either DNA strand, many ...by double strand breaks has been widely used to generate precise changes. However, the efficiency of Cas9...system PE2, which had prime editing efficiencies on average 2.3- to 5.1-fold (though up to 45-fold) higher ...

...cell survival or proliferation, an additional challenge is that only partial or ephemeral knockdown will...from the manufacturer and/or in the published literature. Note that the antibody being published doesn... used in combination with other approaches. If several cell lines express the target at different levels...contains information about the labeling pattern of several cell lines using these two antibodies. This is ...

...Integration. Molecular therapy : the journal of the American Society of Gene Therapy. 2008;16(7):1189-1199...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address...be to prepare them for the arduous process of generating viral particles. Limit tube transfer - the fewer...

...residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP.  The iodine-modifications...cells.  He et al also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed... red fluorescent photosensitizer protein that generates ROS when illuminated with green light. They found...inactivation/cell ablation while having limited collateral damage to bystander cells. Are you ready to use...

...plasmid: name, description, how the plasmid was generated, validated sequence information, and more. The...persistent identification of research reagents, several journals or organizations now recommend or require...authors to use specific PID formats. For example, several journals like eLife, Nature, and Cell use  Research...copy and paste the text attributing the PI who generated the plasmid, followed by the PID(s) of your choice...

...expression, localization, and protein-protein interactions. Beyond these common applications, researchers...when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains flanked...Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation... upon exposure to ultraviolet light. Previous iterations of FLiPs only used N- and C-terminal dronpa fusions...

...of sample preparation for high-throughput next generation (NGS) sequencing have prevented laboratories ...each well of a 96-well plate. Then, samples from several plates are pooled together to create a single library...harder and harder to obtain balanced and sufficient coverage of all samples. The root of the normalization ...important for getting adequate sequencing depth and coverage in order to, for example, assemble hundreds of...

...visualizing genomic loci in living cells. Previous iterations of genomic loci visualization tools using CRISPR...PP7, or boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination... PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from Ma et al., 2016 ... tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

...behind Gram’s crystal violet stain and the ion interactions that cause the stain retention in gram positive...When the negatively charged iodine is added, it interacts with the CV+, creating a complex within the peptidoglycan...trapping the large dye complex created by the interaction of CV+ and I- in the thick multilayered membrane...can be used in medical settings to determine the general type of bacteria present in an infection. This ...