...Rodrigo Arturo Rivera Solís, Universidad Autónoma de Yucatán, Mexico Dr. Rodrigo Arturo Rivera Solís, Associate... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...and to increase the potential for this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR... improving expression of nutritionally and therapeutically significant proteins. In this project she aims...herbicides, such as glyphosate, in water. He aims to leverage a glycine oxidase reaction to indirectly detect...

...possibilities for the clinical and therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs) now...programming spanning from fibroblasts and keratinocytes to peripheral blood mononuclear cells (PBMCs). PBMCs...LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science, 2003... Fukuda, Generation of induced pluripotent stem cells from a small amount of human peripheral blood using... to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood...(Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka...Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work opened up vast possibilities...

... it inspired. CAUTION: Spoilers alert! Pre-DNA Era  H.G. Wells, the father of scientific fiction, was... is now famed as the Griffith’s experiment.  DNA Era The period between 1940-1960 was pivotal for genetics...authors during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the...preference than the ZFNs.   Genetic Engineering Era: Post CRISPR  CRISPR The strategy of using a protein...molecular genetics was booming, SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke,...nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to ...finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can read many base...

...Potential applications for gene and cell-based therapies in the new era of personalized medicine (2017). ...Potential applications for gene and cell-based therapies in the new era of personalized medicine." Genes & diseases...nucleocapsid. B) Three generations of adenoviral vectors: the first generation vectors are deficient in...in trans. The Gutless AdV have generated high interest for gene therapy due their increased cloning capacity...attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate...the Adv genome’s utility in research and its therapeutic potential. Note: constructs discussed are based...based on Adenovirus type 5. Three generations of adenoviral vectors The adenovirus virion is a non-enveloped...

... trusting science in a “fake news and post-truth era,” the politicization of science (from climate change...intellectual prowess and scientific skills. This is an era of social media and self-promotion, and personality...contributed to the steadily increasing distrust the general public has in science and scientists (as discussed...have addressed CRISPR frequently over the last several years, but lay readers may be less familiar with... interesting case studies, and Zimmer provides several. In Part Three of the book, called simply “Old ...

..., a chalk talk is quite simple. It's you, a dry erase board, some markers (chalk is rare these days), ...of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want and you...in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims should have short titles,.../goal, an approach, anticipated outcomes, and preferably a description of the payoff if the aim is successful...bioengineered scaffolds with patient stem cells to generate compatible functional livers.” Why are you the...getting back to your story. Committee questions can derail your talk. You need to balance respectfully answering... for presenting a science talk Check out our transferable skills guide Read all of our career blog posts...

...an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape may be surveyed in unprecedented...present a video reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell Lines ...between deletion size and frequency [3]. There are several applications in which the production of deletions...within the deletion boundaries may be produced to generate clonal allelic series. Single DSBs resolved by...

...colleagues can be more difficult during the pandemic era, with fewer opportunities for spontaneous conversations...creative interaction During Zoom meetings, different tools can be used to promote creative interaction. A great...here, and only general chatter will be allowed with a different host each time that moderates conversations...An example could be making lab meetings more interactive and creating space for discussion at the end ...end of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...also make the screen-sharing experience more interactive. The button is on the right of the Share Screen...writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens et al., 2007...

...methods for C. elegans were released, signaling a new era in C. elegans research. Homology directed repair ...CRISPR in C. elegans even easier - now, one can generate a fluorescent protein fusion, transcriptional ...efficient selection-based CRISPR/Cas9 method to generate knock-ins on any genetic background. Their new...flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC). To make it possible...

...Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes Dis. 2017...is high, it’s generally not as long lasting as AAV (up to several weeks vs. AAV’s several years). But these... To add insult to injury, retroviruses generally have moderate to high immunogenicity, meaning they induce...interchangeable between the two systems, other, more general, viral components such as a heterologous envelope...AAV has many excellent features for human gene therapy and is currently being used in clinical settings...adenovirus have been identified so far! Adenovirus generally has high immunogenicity, but some strains have... fact: All viruses discussed in this blog are generally broadly infective, with one exception. Blood cells...