...turnover rates. Additionally, by engineering FP fusions associated with cellular organelles, scientists...processes, including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although... pitfalls of overexpressed fluorescent protein fusions is to replace the genomic copy of your gene of ...of interest with a fluorescent protein fusion. CRISPR genome editing can help accomplish this goal. The..., allows users to easily create C-terminal tag fusions of endogenous genes in human cells. To use CRISPaint...users to generate a plasmid containing a direct fusion of the desired gRNA to the U6 promoter, and a second...

... create an abasic (empty) DNA site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes...researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE would allow it to act on newly-deaminated...nucleobase hypoxanthine + a ribose sugar. Their fusion of wild-type MPG to an ABE was able to edit A →...al., 2023). gGBE consists only of engineered MPG fused to nCas9. First, MPG removes the G base from DNA...invented. CGBE C → G Base Editor Converts C → G by fusing a uracil DNA glycosylase to a CBE; the first type... H. (2023). Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA...

... neurons and show that smFPs fusions provided better labels than fusions to 3 or more HA tags. 4. High-resolutions...synapses for instance. Also these tags need to be fused to scaffold proteins in order to be expressed stably...contrast to epitope tags, FPs are bigger and their fusion to a POI can affect protein folding and protein-protein...proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags. Indeed, to create the ...difficult to distinguish using standard antibodies. By fusing N-cadherin to smFPs the Looger team was able to...

...complexes, scientists can simply express a bait protein fused to BirA* in cells and then incubate those cells ...and allows scientists to more selectively target fusion proteins to specific locations in the cell.   Find...increased with higher expression levels but for many fusion proteins this would lead to protein/organelle aggregation... is an in vivo technique where two proteins are fused to complementary reporter proteins that can only...molecule that can reassemble to restore activity when fused to two interacting proteins (Schopp et al., 2016...Roux, Kyle J., et al. "A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins...

...epitope tag fusions to a protein of interest. The plasmids used to generate these fusions all use the ...of functions via different fusions. Some applications of PUF protein fusions reported in the literature...leader sequence. Using a variety of GFP and RFP fusions as their test genes, the researchers at the BIOFAB...little as 3 days. Abil et al., 2014 show that you can fuse custom PUF proteins to a translational repressor...

...45 kDa tag which can be purified (along with its fusion protein) with amylose resin. The MBP tag, although...Unlike MBP, the GST tag can be cleaved off from the fusion protein while it is still bound to the glutathione...proper folding of the tag, which prevents insoluble fusion proteins from being purified by GST (maybe try ...ions (Nickel, Cobalt, Copper, etc.), allowing His fusion proteins to be purified by single step metal affinity...affinity chromatography. Purification of His fusion proteins from E. coli is generally more straightforward...

...of plasmids each containing an anti-GFP nanobody fused with a different functional element (tyrosine sulfation...described here can be used to target any protein fused with GFP, YFP, mCerulean, or other related fluorophores... He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX) that labels...plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from a Tet/Shield1 inducible...the 20 nm radius surrounding the dSpyCas9-APEX2 fusion.  This Sontheimer lab used this technology, which...

...stable scaffold, part epitope binding region, all fused to a fluorescent protein. “Frankenbody,” like the...variable fragments (scFvs) (Inbar et al., 1972) are fused to mature fluorophores, and can bind and light up...scaffolds specific to different targets, the scientists fused them to sequences called complementarity determining...of interest tagged with the HA epitope, the lab fused a fluorescent protein to the hybrid anti-HA scFvs...sensitive cells such as living neurons, and can be fused to other fluorescent proteins and tags including...

...protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and...readout, respectively. mNeonGreen can be used as a fusion tag for a wide range of applications from traditional...mCitrine and mOrange or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to...termini of the fluorescent protein and found that the fusion protein localized to their expected locations. ...

...easier - now, one can generate a fluorescent protein fusion, transcriptional reporter, and loss-of-function...template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid...loss-of-function allele as well as a promoter-FP fusion for the gene of interest. L1/L2 larvae from these...phenotype. These animals contain a fluorescent protein fusion for the targeted gene. After initial testing, Dickinson...genome. Although they tested fluorescent protein fusions, this system could be used to make many other genome...