We narrowed to 740 results for: NSI

...fluorescence intensity remains. The first issue is that, in several cases, the fluorescence intensity decay ..., fluorescent proteins may display reversible intensity changes (Shaner et al, 2008; Bindels et al, 2017...experiment is performed that measures fluorescence intensity over time. In order to predict how fluorophores...proteins and by comparing how the fluorescence intensity changes over time, the photostability of the fluorescent...photobleaching rate does not depend on fluorescence intensity or protein distribution. Therefore, photostability...analogous to radioactive decay). Consequently, the intensity decay is often described by the t½, which is the... it is necessary to know how the fluorescence intensity evolves over time. The second issue is that, in...

...help with transitioning out of academia (because so many scientists are making this transition). Another...have experience and knowledge to help scientists transition out of the academic sphere into a different meaningful...not always as effective in mentoring for this transition. Second, a coach will help you set goals and ...successful outcome and the good ones take this responsibility seriously. As I make my rounds speaking at ...However most coaches who focus on scientists are sensitive to this and have coaching plans that are financially...here are a number I have come across for you to consider. Feel free to add other suggestions in the comments...scientific backgrounds. For a stated purpose of transitioning a scientist from academia to industry, you might...

...2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events.... Z. (2024). A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events... tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors kit Antibodies...collaborators recently created ASAP5, a faster and more responsive genetically-encoded voltage indicator (GEVI) ...ASAP5 selected for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and...regimes with fast activation kinetics and high responsivity near the resting membrane potential, ASAP5 is...the formation of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc...

... problem. It is estimated that mycoplasma is responsible for up to 60% of the cell culture contamination...contamination (Uphoff, 2002). Mycoplasmas are considered to be one of the simplest and smallest bacteria. The absence... source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination Detection...Drexler, 2011). Culturing on agar plates/broth is considered the ‘gold standard’ for detecting mycoplasmas...noticeable color change. This method has high sensitivity but low specificity (Degeling, 2012). Bioluminescence...result interpretation might be difficult. The low density of mycoplasmas, contamination by other bacteria...you keep all the required equipment and reagents inside the laminar airflow before and while you are working...

...Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293-EBNA1 Cells. Methods Mol...based on magnetic beads from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-...At its core, harvesting recombinant antibodies consists of separating antibodies from media, cells, and...this method, beads are incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet...alongside your ladder and a protein standard. The density of the antibody bands can be measured against the...multiple contaminating bands or a few strong ones, consider increasing the number of washes performed during...conformational changes in the antigen or lower detection sensitivity of the assay. Where possible, it can be useful...

...system both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance...system. These CRISPR antimicrobials successfully resensitized a beta-lactam resistant population to the antibiotic... may result in plasmid loss and antibiotic resensitization, or may also cause cell death. Image from Beisel...et al. designed a two-phage CRISPR system to resensitize antibiotic-resistant bacteria (Figure 2). The...Bacteria that receive this phage are now antibiotic sensitive, but lytic phage-resistant. When the lytic phage...killed, antibiotics can be used to target the sensitive population. Yosef et al.’s proof-of-concept work....   Figure 2: Two-phage strategies to resensitize bacteria to antibiotics. A lysogenic phage infects...

...in yeast with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive fluorescent biosensor-based...-emission biosensor Rosella consists of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 ...Virus (WNV) induced cell death. The Wu lab library consists of 77,406 individual gRNAs targeting a total of...Moduclar Cloning (CIDAR MoClo) Parts Kit. This Kit consists of a variety of plasmids containing many different...plasmids to the scientific community. This collection consists of plasmids containing genes from a crowd-sourced...scientists use GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes ...all under the control of the cI857 temperature-sensitive repressor. The temperature-controlled expression...

...also incorporated LOV-Turbo into TransitID experiments.  Find TransitID plasmids here! These two developments...genetic cargo Optogenetic proximity labeling and TransitID Tracking cellular history with protein ticker ...37612276.   Optogenetic proximity labeling and TransitID By Mike Lacy Proximity labeling can identify protein...utility of this technique.  By engineering a light-sensitive LOV domain into the TurboID enzyme, Lee and Cheah...plasmids here! Next, Qin and Cheah et al. described TransitID, where two proximity labeling enzymes, TurboID...catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential labeling by TurboID and APEX2 ...trafficking within and between living cells by TransitID. Cell, 186(15), 3307–3324.e30. doi: 10.1016/j....

...Cell lines should be routinely subcultured at densities that are low enough to prevent confluency but ... in lower lentiviral titers. Another factor to consider is the age of your culture post thaw. In general...can be subtle and may include reduced saturation density, decreased rates of growth, or clumping. To ensure... and resource consumption due to the high cell density of the culture. In addition to benefitting your...lower titer virus. In such cases you may want to consider concentrating your viral prep. Storage can also... start to a successful infection, one point to consider is how the virus is being titered. Most titering... that routinely produce lentivirus may want to consider testing a variety of cation and serum sources ...

...experiments a bit more challenging. With these considerations in mind, when planning an experiment users ...transgene, and downstream applications should all be considered when choosing the correct system. Recently, ...volume. Consequently, you will end up with a considerably lower volume than with the unconcentrated prep...experiment requires a certain volume of virus, consider scaling up by either increasing the number of ...while the PEG precipitation method is relatively inexpensive and does not require an ultracentrifuge, PEG ...lentivirus, it is time to titer. One important consideration when titering a lentivirus is the cell line ...transducing a target cell line. One should also consider the specifics of the titering transduction experiment...