...Even if you don’t get the specific position he or she originally called about, you can keep in touch and...

...Lee is a graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage...

... bacteriology and drug resistance. Additionally, she is passionate about health equity, science communication...Antibiotics 3. WHO Antimicrobial Resistance Fact Sheet  Additional Resources on the Addgene Blog Learn...

...National Emerging Infectious Disease Laboratories.  She is interested in genome replication and transcription... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional...

...proteins for subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University ...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5...

...addition to helping with daily laboratory operations, she also works on improving laboratory efficiency through...samples can be stored in? Different shelves? Different racks on each shelf? Give each container a specific...samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received as DNA...large collections. Many companies, such as Thermo Fisher and Micronic, provide 2D barcoded tubes. 1D barcodes...done easily with a barcode reader and an Excel spreadsheet. Start by scanning the sample barcodes into the...and use this ID to name the corresponding Excel sheet.3. Be specific  - When assigning a physical location...

...Annette Sievers, Ph.D., for her tips and guidance. She is way better at protein purification than he is....

...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up in post...hopefully they’ll be just as interesting as the finished product for you, the viewer. While you’re here...

...that control who a female wants to mate with and if she decides to raise their offspring or leave them to... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because...

...only a princess who suffered a terrible fate when she pricked her finger on the spindle of a spinning wheel...Groucho Marx, so the gene was aptly named groucho (Fisher and Caudy, 1998). Interested in plasmids that contain...

... Excellence in Molecular Plant Sciences in 2019. She joined Sichuan Normal University and became a lecturer...Originally published Apr 13, 2021 and last updated Aug 12, 2021. This post was contributed by guest bloggers...University, Jieze Zhang, an intern in the Yang Lab, and Sheng Yang, a professor in synthetic biology from the ... optimizing strains with low efficiency.       Sheng Yang obtained his Ph.D. from Shanghai Institute ...biomedical applications.   Yiwen Zhang entered the Sheng Yang Lab of the CAS Center for Excellence in Molecular...

...with a PhD in reproductive biology and genetics. She loves to communicate the latest and greatest in scientific...companies supply gRNA sequences, such as GenScript, ThermoFisher Scientific and OriGene, or you can order them...

...systems in the Sashital Lab, Iowa State University. She is actively interested in science communication and...in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK. Two-step-SHERLOCK...sequences, Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves...2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter...sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in the femto-aptomolar range...light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies in the sequence...JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic acid detection with CRISPR nucleases....

...Systems Biology department at Harvard University. She is a scientist with a passion for photosynthetic ...

... writer at the Institute for Protein Innovation. She writes about all things IPI, from the scientists ...all bind outside the ligand-binding pocket, their sheer size impeding ligand attachment. The five other ...recommended for use in flow cytometry. Based on the established applications of the original hybridoma monoclonals...

...Morgridge Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of...

... real-world impacts of health services research. She is particularly interested in driving scientific ...

...your research and choose wisely. From the Tenure She Wrote blog, Toxic Academic Mentors.  What is workplace...the PI? Or do lab alumni leave the field or get pushed out by the PI? What is the management style? How...

...Laboratory Medicine at the University of Washington. She is particularly interested in science communication...

...currently a doctoral candidate at Binghamton University. She works on genome engineering and cancer immunotherapy...

...30% of applicants received sponsorship) then he or she can start working in October of the same year. The... to obtain permanent residence. The recently established case Matter of Dhanasar provides the three-prong...demonstrate outstanding achievement Evidence of published material in professional publications written ...

...Pharmacological Sciences, a Cell Press reviews journal. She joined Cell Press to pursue a career in science communication...

...plasmid(s) online (left), choose to submit published or unpublished plasmid(s). Fields marked with an asterisk...submitting plasmid(s) using a spreadsheet (right), click the “Download Deposit Spreadsheet” button to download ... the online form you can submit "Published plasmids" or "Unpublished or pre-publication plasmids" and ...plasmids. You can submit "Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that...(for online submission) or "Download Deposit Spreadsheet" (for emailed submission) in the Deposit menu..."Submit Online Deposit" or "Download Deposit Spreadsheet". Start your plasmid deposit There are two options...Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered using...

...Gordon Fishell New viral prep pAAV_BiSSTe10_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiVIPe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiPVe3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiSSTe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...

...deeper imaging into thick tissues. Light Sheet Microscopy Light Sheet Microscopy typically utilizes a configuration...evanescent energy field. Lightsheet microscopes that project ultra-thin sheets of excitation light through...tissues need to be imaged via Lightsheet microscopy. Although not all lightsheet microscopes can cover samples...1); similar to an MRI or CT scanner. This is accomplished by focusing excitation light to a point in the... bleaching) to a single focal point. This is accomplished by using, for example, red light instead of ...configuration of two or more objectives to create a thin sheet of excitation light that propagates perpendicular...sectioning (5). One such example is the lattice lightsheet that projects a number of thin cone-shaped beams...

...sharing policy. Since early March, the open access publisher has required authors to include a note as to where...result of consultations between researchers and publishers. Nonetheless, the initial release caused a storm...increasing the ability of reviewers (and, once published, anyone) to detect possible errors in assumptions...their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel tools and... video, generating huge amounts of data. When published, this data is usually condensed into a statistically... data are likely to improve research. What is published is typically a tiny fraction of what was actually...data accessibility and methodological detail. Publishers have also contributed to this issue by requiring...

...research project, the vast majority of what gets published in scientific journals flies under the radar and...have time to keep up with all the research being published on our own topic. Only on occasion do we then ...   Video abstracts about research, especially published research, have grown in popularity over the last...this: “In terms of total view count and videos published, YouTube reported a total of 865,995 views for...online article downloads – especially if they are published in open access journals.   Check Out Addgene Videos...Facebook is continuing its growth, and while many publishers and researchers have Facebook pages, few share... leading the way, the future of complementing published research with video overviews was growing quickly...

...Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Gordon Fishell   Optogenetics Optogenetics continues...Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid...pAAV-mDlx-GFP-Fishell-1 Biosensors Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 ...

...and an ever increasing number of journals and publishers to choose from, it gets very confusing for scientists...research may stipulate that your article must be published in an open access journal or by using the open...your manuscript will be available to anyone who wishes to read it, without them needing a subscription...data resulting from their funding should also be published. All these factors will influence your choice ...manuscript needs to be revised before it can be published, this can delay publication and manuscripts may...referee report are permanently attached to the published article. In addition, authors and readers are ...help your chances of getting your manuscripts published in the future. Disregard reviewer’s comments without...

...of the number of papers with your search term published each year, which is a nice way to see growth or...receive a daily, weekly, or monthly digest of newly published papers that contain that term. You can also set... a collection, which is essentially a virtual bookshelf. Pro tip The “cited by” and references sections...papers related to a particular topic or that were published by the lab down the hall.  Scientific journals...graph of the number of papers with your key term published each year is displayed with your search results...searches to particular authors, with the authors who published the most papers with your keyword populated at...Meta. Feeds automatically update as papers are published, similar to Facebook or Instagram. Key features...