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We narrowed to 312 results for: Mut

Showing: 161 - 200 of 312 results

  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...outlined below (or emission wavelenght range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP)...breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's post from last week on GFP....

  3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors two surface exposed...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...

  6. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... but won’t tell you if your products have any mutations.  There are lots of different cloning strategies...expected sized PCR product doesn’t mean there aren’t mutations in your insert. Make sure to submit multiple positive...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...the deletion reduces the risk of insertional mutagenesis by limiting the interaction between viral LTR...potential for oncogenesis through insertional mutagenesis. These risks are largely based upon the vector...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...HaloTag, Figure 4), they determined that an A122D mutation in the rhodopsin domain conferred several new ...potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also...

  9. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  This process...For example, multiple studies show that certain mutations of the BAP1 gene play a role in susceptibility...

  10. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...determine the presence and quantity of DNA, RNA or a mutation of interest. Together SHERLOCK and DETECTR demonstrate...SHERLOCK could also detect low-frequency cancer mutations from cell-free DNA fragments as well as health-related...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... HEPN domains in other proteins. As with Cas9, mutating key residues in the Cas13a molecule results in...Zika virus, genotype human DNA, identify tumor mutations within cell-free genomic DNA, and detect COVID...

  13. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein homologues...

  14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...2013) Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas...Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922...

  15. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...mEGFP. This variant was made by introducing two mutations (F64L, S65T) in the original jellyfish GFP (AvGFP...with improved brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate the strictly ...

  17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ... spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves methylation kinetics such that cytosine...fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility, and this...

  18. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ...

  19. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...active area of research due to the possibility that mutation or recombination with human retroviruses could...Efficient gene transfer Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human...

  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...with senior investigators. Discussions can reveal mutual scientific interests, complementary perspectives...research to search committees, and can lead to mutually beneficial collaborations. 7. You cannot get too...

  21. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... into question. Specifically, CRISPR-generated mutants believed to be knockouts can maintain partial function...process is tedious and time consuming, as RNAi mutants with a reduced growth phenotype will require additional...

  22. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses... Russell, David W. "AAV vectors, insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007):...

  26. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...multiple genes in a cell population. The resulting mutant cells are screened for phenotypes of interest. ...

  27. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ... new tool for making diverse, localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid...

  28. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV9 Biosensors (Control) Li New viral service...

  29. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    .... Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases the number of...

  30. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...DNA strand. The lab then found gain-of-function mutations that allows it to be repurposed for double strand...

  33. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...Single base editor that induces C-to-T and A-to-G mutations Nozomu Yachie’s lab recently developed a base ...

  37. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...

  38. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...useful to me as some days it was not as easy to commute as others. I know some fear that remote work would...

  40. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...other hand, viruses can evolve to evade CRISPR by mutating these sequences. Pro tip! Various labs have created...
Showing: 161 - 200 of 312 results