We narrowed to 318 results for: Mut

...promoter. The resolution step leads to the desired mutation and excision of the vector backbone. To do this...Characterization of a Blue Chromoprotein and Its Yellow Mutant from the Sea AnemoneCnidopus Japonicus. Journal...

...due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability and yield. If a plasmid...using older plates could increase plasmid loss or mutation. Let it incubate Increasing the incubation time...

... E. The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations. Science...

...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

...outlined below (or emission wavelenght range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP)...breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's post from last week on GFP....

...which contains multiple point mutations in the nuclease domain. These mutations result in an inactive RNA-...

...et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors two surface exposed...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...

...success of BiFC experiments. The ideal control is a mutated variant of one or both of your PIPs in which the...requires that you empirically test a number of mutants to identify those that inhibit BiFC in your cellular...

... but won’t tell you if your products have any mutations.  There are lots of different cloning strategies...expected sized PCR product doesn’t mean there aren’t mutations in your insert. Make sure to submit multiple positive...

...the deletion reduces the risk of insertional mutagenesis by limiting the interaction between viral LTR...potential for oncogenesis through insertional mutagenesis. These risks are largely based upon the vector...

... HEPN domains in other proteins. As with Cas9, mutating key residues in the Cas13a molecule results in...Zika virus, genotype human DNA, identify tumor mutations within cell-free genomic DNA, and detect COVID...

...inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  This process...For example, multiple studies show that certain mutations of the BAP1 gene play a role in susceptibility...

...HaloTag, Figure 4), they determined that an A122D mutation in the rhodopsin domain conferred several new ...potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also...

...determine the presence and quantity of DNA, RNA or a mutation of interest. Together SHERLOCK and DETECTR demonstrate...SHERLOCK could also detect low-frequency cancer mutations from cell-free DNA fragments as well as health-related...

...methods were available to create targeted gene mutations in anoles lizards or in any reptiles at all. But...was able to make an albino lizard with targeted mutations. Now what? What would you study first? They are...

... the sequence, normally through the synonymous mutation of a codon. You can domesticate the sites yourself...colony PCR electrophoresis), is free of undesired mutations, and that you have the correct four-base overhangs...

...mEGFP. This variant was made by introducing two mutations (F64L, S65T) in the original jellyfish GFP (AvGFP...with improved brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate the strictly ...

...biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein homologues...

...2013) Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas...Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922...

... can mediate recombination.) The deletions are mutually exclusive, as either deletion removes one of the...following the promoter. The STOP cassette includes a mutant YFP that does not fluoresce, but can be detected...

... spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves methylation kinetics such that cytosine...fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility, and this...

...them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses... Russell, David W. "AAV vectors, insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007):...

...active area of research due to the possibility that mutation or recombination with human retroviruses could...Efficient gene transfer Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human...

... into question. Specifically, CRISPR-generated mutants believed to be knockouts can maintain partial function...process is tedious and time consuming, as RNAi mutants with a reduced growth phenotype will require additional...

...with senior investigators. Discussions can reveal mutual scientific interests, complementary perspectives...research to search committees, and can lead to mutually beneficial collaborations. 7. You cannot get too...

... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ...

...especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence ...doi.org/10.1038/s41592-021-01338-6  Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C.,...

...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...

... new tool for making diverse, localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid...

...multiple genes in a cell population. The resulting mutant cells are screened for phenotypes of interest. ...

... focusing on safety and the ability to generate mutant microbial strains faster than ever before. 29:54...

...viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV9 Biosensors (Control) Li New viral service...

.... Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases the number of...

... protein’s structure and also discovered many mutations that alter and improve its function and shift ...

...DNA strand. The lab then found gain-of-function mutations that allows it to be repurposed for double strand...

...infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA specific of a viral sequences...

...Single base editor that induces C-to-T and A-to-G mutations Nozomu Yachie’s lab recently developed a base ...

...pathways, they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes...

...discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are lethal, associated with defective...

...adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and nLightR uses...