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We narrowed to 602 results for: ACE

Showing: 201 - 250 of 602 results

  1. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ... and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting Tenure...thing to do is not go into their labs in the first place. The good news There are many excellent mentors ...

  3. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...tech companies using only laptops and some server space. But what if you want to be an entrepreneur in ... graduate students with innovative ideas in all facets of the entrepreneurial process in order to develop...you start at a disadvantage in the health science space if you don’t have an advanced degree behind your...

  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a.m. many ...format I decided on was to have two teams of 4 people face each other on a single chosen clinical disease case...they agreed on each element to use, they had to place the selected card on the board game. They had 35...

  5. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... that cell. Since there is a limited amount of surface area on each cell, the way to get more AAV in contact...expression. How do you achieve high titers in the first place? Some AAVs are known to be difficult to produce ...concentration filter column, make sure to rinse off the surface of the filter membrane with the surrounding solution...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it becomes... which is freely available online. Its simple interface can guide you through both experimental and primer...

  8. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...solution thawed, as the CO2 was confined to the tube headspace when the samples were frozen. This was clear by...the gradual change in pH visible at the sample interface upon thaw (Fig. 2). Before thawing, all the samples...tubes prior to thaw released the CO2 from the headspace, thus mitigating the pH effects of dry ice storage...

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...

  10. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...complexity of antigen processing events that take place within an antigen-presenting cell like a T cell....

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs developed...discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4...genes identified as non-essential. The knockouts replace entire protein-coding regions (excluding start ...

  12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target virtually...either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown...dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT exon 10 splice acceptor...

  13. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...genetic backgrounds. The standard RNAi vector, L4440, places a target gene between two inverted T7 promoters...’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...it is essential to contain outbreaks when they surface. But detection of the virus costs time and money...

  14. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...One of the most pivotal decisions many PhDs face when transitioning from academia to industry is whether...requires deliberate preparation and a willingness to embrace new responsibilities. After my second postdoc,...permission to explore, reflect on your priorities, and embrace these transitions as an integral part of your broader...

  15. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...substrate, they synthesized diacetyl coelenterazine-h. Unlike coelenterazine-h, diacetyl coelenterazine-h does... to the media. Cellular esterase then converts diacetyl coelenterazine-h to coelenterazine-h, producing...

  16. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...guided by a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...

  17. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...cell!)—ATP. There are biosensors for intracellular, extracellular, and even the ADP:ATP ratio. Most of... from dopamine to glutamate, all the way to acetylcholine. These sensors have been tested in vivo, in ...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...forms tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage...predicted with no addition of nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either...once, and each <200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will facilitate...

  19. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ... There is essentially no better place for a scientist to make new relationships than at scientific conferences...rut of hanging out with only your lab mates (let’s face it, you see enough of them).  Before the conference...students and postdocs. There are many inexpensive/free places to order cards. There are a few good reasons to...

  20. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...biggest difficulties that plant biotechnologists face is public perception of genetic modification (GM...and we’d love to hear about the obstacles you’ve faced as a plant scientist, and how you were able to overcome...Bioengineering Follow RevBio on Twitter Like RevBio on Facebook Read more Addgene blog posts about synthetic...

  21. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under the control of...needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a constitutive promoter...production titers decreased. But when the genes were placed under the control of a stabilized promoter, deoxychromoviridans...

  22. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...quicker to pick up new languages!) Jargon has a place in science communication, but the more you use, ...of detail Again, this is an obvious one on the surface, but it’s quite easy to add in “necessary” detail...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed. The ...

  23. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were even ...into M phase oocytes, thus allowing repair to take place before DNA replication. Indeed, they observed that...articles and commentaries) pop up on Twitter and Facebook, shared by biologists and non-biologists alike...

  24. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...talked with Voigt about this collision that’s taking place between CRISPR technology and synthetic biology,.... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental... Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 ...

  25. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant...version of which will feature a simplified user interface, expanded numbers of curated molecules, including...over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely...

  26. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ... like cliff notes, so I use these as a starting place for drafting my stories. In total, I prepare 5-6...arrangements with those you live with to have a quiet place to take your video call.  Expect technical difficulties...

  27. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...and recurrent infections along with a mild or exacerbated inflammatory response which leads to nearly 25%...inhibits a potassium channel, causing release of acetylcholine. Given its unique molecular biology and previous...pathophysiological role of glycans. Follow her on Facebook: @glycoimmunologygroup.     Michael McMurray is...

  28. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...experimenter picks up a mouse from the home cage and places it into the experimental setup, the mouse becomes...Both AAV and lentiviral vectors are used for intracerebral injections. AAV particles are smaller and may...liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue damage; however, the...

  29. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...indicated by exclamation marks. Error points include places where DNA is being created, such as PCR amplification...request the lab provide a new, sequence-verified replacement for the plasmid, and the full QC process is started... started over again after receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s ...

  30. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...for you in one place! First, a quick refresher: prime editing is a “search and replace” genome editing..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

  31. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...module entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...

  32. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

  33. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the virus to enter ...in the molecular crevices proteins on the cell surface. Figure 1: Comparison of common antibody,... is tricky because they have fewer hydrophilic surfaces, require detergents or lipids to remain folded...

  34. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...definitive, but visual identification is a good place to start before taking on more time consuming and...fitness cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using...Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine with hydrophilic...

  35. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...formation of disulfide bridges, glycosylation and acetylation to create functional, stable proteins. Protein...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose...

  36. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...but it actually requires more technical skill to place the needle in the correct vein without collapsing...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

  37. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...Korte et al., 2021) for all disposable items and surfaces that come into contact with the rAAV and apply...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  38. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing.... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

  39. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type...produces the phenotype you screened for in the first place. With the proper experimental design and validation...

  40. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... who need to overexpress and purify proteins can face any number of technical challenges depending on ...protease. A TEV protease cleavage site is frequently placed between two tags being used for two rounds of purification...purification, with the cleavage reaction taking place between column runs. The TEV protease itself, with...

  41. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...focused on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA...RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts of this disease.  As a...is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought together a strong...

  42. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...fluorescent proteins might not localize to the right places, which can provide misleading information on where...

  43. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...conference can be intimidating. And it can be exacerbated when everyone seems to know one another, but ...program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring circles We looked at...this first year. Mentors Amanda Gunn, Bryan Gibb, Candace Williams, Dave Baltrus, Geof Hannigan, Irene Newton...

  44. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...overexpressing mice spent more time in the risky bright place, driven by their motivation to explore novel environments...treatment in rodents. In this test, an animal is placed into a tank of water with no support from below...attempts to escape, or passive floating on the water surface. The latter reflects a state of behavioral despair...

  45. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...recently moved to our brand new location, with more space to grow and help scientists share resources in new...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments of the...actually clump together forming one patch on the surface, giving the embryo a “spiny and prickly look” that...

  46. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...frequently why many people get into science in the first place. The feeling of camaraderie one gets from a well-functioning...could dread these interactions, but you could also embrace them. Talking about science to non-scientists can...curiosity that led you into science in the first place. Finally, if you still find the idea of not holding...

  47. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an ...-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in performance...www.janelia.org/open-science/voltron-and-positron.  Both Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent...

  48. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development, ...GenBank, but it was useful to present it all in one place to get a sense of the similarities and differences...

  49. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... and excision recombination reactions that take place when lambda phage infects bacteria. In vivo, these...reactions described below: The BP Reaction takes place between the attB sites flanking the insert and the...gene expression.     The LR Reaction takes place between the attL sites of the generated entry clone...

  50. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...science. Facebook is continuing its growth, and while many publishers and researchers have Facebook pages...article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science, video...
Showing: 201 - 250 of 602 results